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Embryo aggregation does not improve the development of interspecies somatic cell nuclear transfer embryos in the horse
(Elsevier Science Inc, 2016-09)
The low efficiency of interspecies somatic cell nuclear transfer (iSCNT) makes it necessary to investigate new strategies to improve embryonic developmental competence. Embryo aggregation has been successfully applied to ...
Embryo Aggregation in Pig Improves Cloning Efficiency and Embryo Quality
(Public Library of Science, 2016-02)
In this study, we analyzed the effects of the cloned embryo aggregation on in vitro embryo development and embryo quality by measuring blastocyst diameter and cell number, DNA fragmentation levels and the expression of ...
The aggregation of four reconstructed zygotes is the limit to improve the developmental competence of cloned equine embryos
(Public Library Of Science, 2014-11)
Embryo aggregation has been demonstrated to improve cloning efficiency in mammals. However, since no more than three embryos have been used for aggregation, the effect of using a larger number of cloned zygotes is unknown. ...
Production of chimeric embryos by aggregation of bovine egfp eight-cell stage blastomeres with two-cell fused and asynchronic embryos
(Elsevier Science Inc, 2013-06)
Embryo disaggregation allows the production of two to four identical offspring from a single cow embryo. In addition, embryo complementation has become the technique of choice to demonstrate the totipotency of embryonic ...
Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression
(Bioscientifica, 2015-07)
The aim of this study was to evaluate the capacity of domestic cat (Dc, Felis silvestris) oocytes to reprogram the nucleus of cheetah (Ch, Acinonyx jubatus) cells by interspecies SCNT (iSCNT), by using embryo aggregation. ...
State of the art on animal embryonic chimeras
(2013)
Embryonic chimerism is generally used in basic research and in vivo diagnosis of undifferentiated embryonic stem cells (ESC), mostly using mice embryos, although there have been reports in the literature on using rat, ...
In Vitro Development and Cell Allocation After Aggregation of Syngeneic Wild Type and Fluorescence-Expressing Bovine Cloned Embryos
(UNIV FED RIO GRANDE DO SULPORTO ALEGRE RS, 2012)
Background: The in vitro production (IVP) of embryos by in vitro fertilization or cloning procedures has been known to cause epigenetic changes in the conceptus that in turn are associated with abnormalities in pre- and ...
Screening of biotechnical parameters for production of bovine inter-subspecies embryonic chimeras by the aggregation of tetraploid Bos indicus and diploid crossbred Bos taurus embryos
(2016-03-01)
The aggregation of a tetraploid zebu embryo (Bos indicus, a thermotolerant breed) with a diploid taurine embryo (Bos taurus, a thermosensitive breed) should create a complete taurine fetus, whose extra-embryonic components, ...
In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence
(FUNPEC-EDITORA, 2010)
The relationship between the level of cell confluence near the plateau phase of growth and blastocyst yield following somatic cell cloning is not well understood. We examined the effect of distinct cell culture confluence ...