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Revisando os efeitos da concentração de oxigênio sobre o desenvolvimento embrionário
(2012-01-01)
Despite the fact that the processes of embryo development and implantation of mammals do not occur in atmospheric tension in vivo, it is common practice in laboratories for in vitro fertilization (IVF) the culture of embryos ...
Effects of different cryopreservation methods on post-thaw culture conditions of in vitro produced bovine embryos
(Cambridge Univ PressNew York, 2012-05)
The aim of this work was to evaluate the effect of cryopreservation protocols on subsequent development of in vitro produced bovine embryos under different culture conditions. Expanded in vitro produced blastocysts (n = ...
Changes in soluble carbohydrates and starch amounts during somatic and zygotic embryogenesis of Acca sellowiana (Myrtaceae)
(SPRINGER, 2008)
Comparative analysis of zygotic and somatic embryogenesis of Acca sellowiana showed higher amounts of sucrose, fructose, raffinose, and myo-inositol in zygotic embryos at different developmental stages than in corresponding ...
In Vitro Propagation of Heliconia bihai (L.) L. from Zygotic Embryos
(SOC BOTANICA BRASIL, 2010)
(In vitro Propagation of Heliconia bihai L. from Zygotic Embryos). The internal morphology of embryos from immature and mature fruits of Hcliconia bihai (L.) L. cv. Lobster Claw Two was examined. Embryos were inoculated ...
Sexing of murine and bovine embryos by developmental high-titer arrest induced by H-Y antisera
(Elsevier B.V., 2004-12-01)
Murine and bovine embryos at the late morula stage were cultured in medium containing high-titer rat H-Y antisera. After 12 h of incubation, embryos blocked at the late morulae stage were classified as males and those at ...
Lipid content and apoptosis of in vitro-produced bovine embryos as determinants of susceptibility to vitrification
(Elsevier B.V., 2011-04-15)
The objective was to evaluate supplementation of fetal calf serum (FCS) and phenazine ethosulfate (PES), a metabolic regulator that inhibits fatty acid synthesis, in culture media during in vitro production (IVP) of bovine ...
In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence
(FUNPEC-EDITORA, 2010)
The relationship between the level of cell confluence near the plateau phase of growth and blastocyst yield following somatic cell cloning is not well understood. We examined the effect of distinct cell culture confluence ...
IVF/ICSI outcomes after culture of human embryos at low oxygen tension: a meta-analysis
(Biomed Central Ltd., 2011-11-01)
Background: Improved pregnancy, implantation, and birth rates have been reported after the use of reduced O2 concentration during embryo culture, mainly due to a reduction of the cumulative detrimental effects of reactive ...