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A silver staining method for single-cell gel assayA silver staining method for single-cell gel assay
(SAGE PublicationsSAGE Publications, 2001-12)
The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA ...
Cytoprotective Effect of the Eryngium foetidum “Sacha Culantro” Methanolic Leaf Extract versus Sodium Fluoride Exposed Mice using the Micronucleus Test and the Comet Assay
(Pharmacognosy Network Worldwide, 2019)
Introduction: Eryngium foetidum, commonly known as sacha culantro, is a plant that grows in the Peruvian Amazonian jungle, studied for its high concentration of antioxidant substances and various ethnopharmacological ...
Cytoprotective Effect of the Eryngium foetidum “Sacha Culantro” Methanolic Leaf Extract versus Sodium Fluoride Exposed Mice using the Micronucleus Test and the Comet Assay
(Pharmacognosy Network Worldwide, 2019)
Introduction: Eryngium foetidum, commonly known as sacha culantro, is a plant that grows in the Peruvian Amazonian jungle, studied for its high concentration of antioxidant substances and various ethnopharmacological ...
Genotoxic effects of commercial formulations of Chlorpyrifos and Tebuconazole on green algae
(Springer, 2014-09)
The alkaline single-cell gel electrophoresis assay (comet assay) was used for the study of the genotoxic effects of insecticide Chlorpyrifos and fungicide Tebuconazole (commercial formulations) on two freshwater green algae ...
Detection of excision repaired DNA damage in the comet assay by using Ara-C and hydroxyurea in three different cell types
(Springer, 2009-02)
Because of its characteristics, the comet assay has been used to evaluate the ability of virtually any type of eukaryotic cell to repair different kinds of DNA damage, including double and single strand breaks and base ...
Genotoxicity of glyphosate assessed by the comet assay and cytogenetic tests
(Elsevier Science, 2009-07)
It was evaluated the genotoxicity of glyphosate which up to now has heterogeneous results. The comet assay was performed in Hep-2 cells. The level of DNA damage in the control group (5.42 ± 1.83 arbitrary units) for tail ...
Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
(Taylor & Francis, 2011-08-27)
Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to classify individuals as ...
Comet assay in gill cells of Prochilodus lineatus exposed in vivo to cypermethrin
(Academic Press Inc Elsevier Science, 2013-11)
Agricultural chemicals can induce genetic alterations on aquatic organisms that have been associated with effects on growth, reproduction and population dynamics. The evaluation of DNA damage in fish using the comet assay ...
Alkaline Comet Assay for Genotoxic Effect Detection in Neotropical Fish Prochilodus lineatus (Pisces, Curimatidae)
(Springer, 2009-05)
Toxicants on fish may induce genetic alterations that can be used as genotoxic markers. We evaluated DNA damage using alkaline comet assay applied on erythrocytes after in vivo exposure of Prochilodus lineatus to different ...
Genotoxicity evaluation of the insecticide imidacloprid on circulating blood cells of Montevideo tree frog Hypsiboas pulchellus tadpoles (Anura, Hylidae) by comet and micronucleus bioassays
(Elsevier Science, 2014-06)
Acute toxicity and genotoxicity of imidacloprid (IMI) was evaluated on Hypsiboas pulchellus (Anura: Hylidae) tadpoles exposed under laboratory conditions. A lethal effect was used as the end point for lethality, whereas ...