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A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
(Blackwell Publishing, 2004-01-01)
Aims: Detection of Xylella fastidiosa in citrus plants and insect vectors.Methods and Results: Chelex 100 resin matrix was successfully standardized allowing a fast DNA extraction of X. fastidiosa. An amplicon of 500 bp ...
Dependence of divalent metal ions on phosphotransferase activity of osseous plate alkaline phosphatase
(Elsevier B.V., 1997-04-01)
Kinetic evidence for the role of divalent metal ions in the phosphotransferase activity of polidocanol-solubilized alkaline phosphatase from osseous plate is reported. Ethylenediamine tetreacetate, 1,10-phenanthrolin, and ...
Comparación de métodos de lisis y extracción de ADN de trofozoiteos de Giardia lambliaComparison of lysis methods and DNA extraction of Giardia lamblia trophozoites
(Sociedad Chilena de Parasitología, 2006-12)
The efficiency of lysis procedures and DNA purification treatments of Giardia lamblia trophozoites were evaluated regarding the breaking-up efficiency, quantity and pureness of DNA, processing time and costs. Five lysis ...
Determination of in situ speciation of manganese in treated acid mine drainage water by using multiple diffusive gradients in thin films devices
(2013-10-17)
Acid mine drainage (AMD) is a serious environmental problem that creates acidic solution with high Mn concentrations. The speciation of residual Mn from AMD after an active treatment involving the addition of a neutralizing ...
In situ redox speciation analysis of chromium in water by diffusive gradients in thin films using a DE81 anion exchange membrane
(2016-07-01)
A method for the in situ redox speciation analysis of chromium in water by the diffusive gradients in thin films (DGT) technique using a DE81 anion exchange membrane was successfully developed. For the selective uptake of ...
In situ speciation of uranium in treated acid mine drainage using the diffusion gradients in thin films technique (DGT)
(2017-02-01)
The exchange membranes P81 and DE81 and Chelex-100 resin were used to perform in situ speciation of uranium in treated acid mine drainage at the Osamu Utsumi mining site, Poços de Caldas city, Southeast Brazil. To investigate ...
Determination of labile barium in petroleum-produced formation water using paper-based DGT samplers
(Elsevier B.V., 2012-10-15)
A polyacrylamide hydrogel containing the Chelex-100 resin has traditionally been used as the binding agent for the diffusion gradients in thin films (DGT) technique. The Chelex-100 resin, although important for the ...
Determination of labile barium in petroleum-produced formation water using paper-based DGT samplers
(ELSEVIER SCIENCE BVAMSTERDAM, 2012)
A polyacrylamide hydrogel containing the Chelex-100 resin has traditionally been used as the binding agent for the diffusion gradients in thin films (DGT) technique. The Chelex-100 resin, although important for the ...
Extracting DNA of nematodes communities from Argentine Pampas agricultural soils
(Academia Brasileira de Ciencias, 2015-06)
We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an ...
Characterization of the phosphatidylinositol-specific phospholipase C-released form of rat osseous plate alkaline phosphatase and its possible significance on endochondral ossification
(Kluwer Academic Publishers, 1995-11-22)
Alkaline phosphatase activity was released up to 100% from the membrane by incubating the rat osseous plate membrane-bound enzyme with phosphatidylinositol-specific phospholipase C. The molecular weight of the released ...