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Image-based Quantification of Direct Cell-to-cell Transmission of Bovine Viral Diarrhea Virus
(Bio-protocol, 2019-08)
Different viruses rely on direct cell-to-cell transmission to propagate infection within the infected host. Measuring this mode of transmission in cultured cells is often complicated by the contribution of cell free viruses ...
Magnetic-Activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations
(Humana Press, 2018)
This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, ...
Cell-to-cell transmission is the main mechanism supporting bovine viral diarrhea virus spread in cell culture
(American Society for Microbiology, 2019-01)
After initiation of an infective cycle, spread of virus infection can occur fundamentally in two different ways: (i) viral particles can be released into the external environment and diffuse through the extracellular space ...
Growth and Maintenance of Human Giant-cell Bone Tumors (Osteoclastomas) in Continuous Culture
(Karger, 1970)
Tissue culture of six human giant cell tumors of bone was undertaken (one on two occasions). All of them were cultivated successfully. Five cultures have shown outgrowth in about 25 days. In the remaining two cases continuous ...
Simplified microenvironments and reduced cell culture size influence the cell differentiation outcome in cellular microarrays
(Springer, 2013-01)
Cellular microarrays present a promising tool for multiplex evaluation of the signalling effect of substrate-immobilized factors on cellular differentiation. In this paper, we compare the early myoblast-to-osteoblast cell ...
Suspension-Vero cell cultures as a platform for viral vaccine production
(Elsevier, 2009-10)
Since Vero cells are currently considered as an acceptable cell substrate to produce a wide range of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in serum-free media. ...
A simple strategy for culturing morphologically-conserved rat hypothalamic tanycytes
(Springer, 2017-08)
Hypothalamic tanycytes are specialized bipolar ependymal cells that line the floor of the third ventricle. Given their strategic location, tanycytes are believed to play several key functions including being a selective ...
Strategy for erythroid differentiation in ex vivo cultures: Lentiviral genetic modification of human hematopoietic stem cells with erythropoietin gene
(Soc Bioscience Bioengineering Japan, 2017-11)
If cultured in appropriate conditions, such as supplementing culture media with costly cytokines and growth factors, hematopoietic stem/progenitor cells (HSPCs) from different origins have shown to be an adequate source ...
Characterization of cellular states of CHO-K1 suspension cell culture through cell cycle and RNA-sequencing profiling
(Elsevier Science, 2018-11)
Chinese hamster ovary (CHO) derived cell lines are the preferred host system for the production of therapeutic proteins. The aim of this work was to explore the regulation of suspension-adapted CHO-K1 host cell line ...
A long-term in vitro culture of bovine epithelial cells on collagen rafts
(Wiley Blackwell Publishing, Inc, 2021-02)
In the present work, we established and characterized a 3D functional polarized primary bovine oviduct epithelial cells (BOECs) culture on free-floating type I collagen hydrogels (rafts) at an air-liquid interface (ALI). ...