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Detection of infectious bronchitis virus in experimentally infected chickens by an antigen-competitive ELISA
(2001-02-07)
An antigen-competitive enzyme-linked immunosorbent assay (Ag-C-ELISA) was developed for the detection of infectious bronchitis virus (IBV) antigens, M41 strain, in tissues from experimentally infected chickens, or in ...
Evaluation of an indirect ELISA method for the detection of chicken antibodies against infectious bronchitis virus
(1996-01-01)
An indirect ELISA method was developed and applied to detect antibodies against infectious bronchitis virus (IBV). Three hundred serum samples collected from vaccinated and non-vaccinated chickens under field conditions ...
A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus
(Associação Brasileira de Divulgação Científica (ABRADIC), 1999-06-01)
A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector ...
Humoral and cell-mediated immune responses to different doses of attenuated vaccine against avian infectious bronchitis virus
(2013-08-01)
The antibody and cellular immune responses against infectious bronchitis virus (IBV) were evaluated at mucosal sites of chickens after immunization with various doses of an attenuated vaccine at 1 day of age. The correlation ...
Evaluation of serum- and animal protein-free media for the production of infectious bronchitis virus (M41) strain in a continuous cell line
(2005-10-31)
Infectious bronchitis virus (IBV) is produced in vitro using specific pathogen free chicken embryos, primary chicken kidney cells (CKC) and/or tracheal organ culture (TOC). Regulatory authorities in Europe (EMEA) and in ...
Infectious bronchitis virus: detection and vaccine Strain differentiation by semi-nested RT-PCR
(Fundação APINCO de Ciência e Tecnologia Avícolas, 2005-03-01)
A semi-nested reverse transcription-polymerase chain reaction (Semi-N-RT-PCR) was developed and used to detect the S glycoprotein gene of infectious bronchitis virus (IBV) strains and to discriminate H120 vaccine strain ...
Genetic grouping of avian infectious bronchitis virus isolated in Brazil based on RT-PCR/RFLP analysis of the S1 gene
(Colégio Brasileiro de Patologia Animal - CBPA, 2008-03-01)
Doze isolados de campo do Brasil e uma estirpe de referência vacinal do vírus da bronquite infecciosa das aves (VBI) foram propagadas em ovos embrionados SPF. O gene S1 dessas amostras foi analisado por RT-PCR seguido de ...
Development and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against infectious bronchitis virus
(Amer Soc Microbiology, 2005-04-01)
A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay ...