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Assessment of the Effect of Triton X-114 on the Physicochemical Properties of an Antibody Fragment
(American Chemical Society, 2014-05)
The effect of Triton X-114 on the physicochemical properties of a single-chain antibody fragment (scFv) has been studied. According to the far UV circular dichroism spectroscopy, the secondary structure of the recombinant ...
Single-chain antibody fragments: Purification methodologies
(Elsevier, 2013-06)
At present, single-chain variable fragments (scFv) of antibodies are considered one of the most important tools in human therapies. Wide applications of antibodies are being exploited in different medical, pharmaceutical ...
F(ab')2 antibody fragments against Trypanosoma cruzi calreticulin inhibit its interaction with the first component of human complement
(Sociedad de Biología de Chile, 2005)
Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface
(Associação Brasileira de Divulgação Científica, 2000)
We describe the expression of an anti-Z-DNA single chain variable region antibody fragment (scFv) on a filamentous phage surface. Four vectors for phage display were constructed. Two of them are able to display multiple ...
F(ab')2 antibody fragments against Trypanosoma cruzi calreticulin inhibit its interaction with the first component of human complement
(Society of Biology of Chile, 2005)
Trypanosoma cruzi calreticulin (TcCRT), described in our laboratory, retains several important functional features from its vertebrate homologues. We have shown that recombinant TcCRT inhibits the human complement system ...
Serrumab: A human monoclonal antibody that counters the biochemical and immunological effects of Tityus serrulatus venom
(INFORMA HEALTHCARENEW YORK, 2012)
In Brazil, the species Tityus serrulatus is responsible for the most severe cases of scorpion envenomation. There is currently a need for new scorpion anti-venoms that are more effective and less harmful. This study attempted ...
Separation of human Fab fragments on negative mode Ni(II)-TREN-agarose chromatography
(Elsevier Sci LtdOxfordInglaterra, 2014)
An unusual cysteine VL87 affects the antibody fragment conformations without interfering with the disulfide bond formation
(Pergamon-Elsevier Science Ltd, 2017-10)
The Cys residues are almost perfectly conserved in all antibodies. They contribute significantly to the antibody fragment stability. The relevance of two natural contiguous Cys residues of an anti-recombinant human-follicle ...