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Photo-induced generation of the riboflavin-tryptophan adduct and a vibrational interpretation of its structure
(1994)
A pure fraction of a riboflavin-tryptophan adduct was obtained for the first time from a mixture of products resulting from the anaerobic photoirradiation of riboflavin and tryptophan in solution. The procedure used to ...
Surface-enhanced Raman scattering study of L-tryptophan
(2009)
Surface-enhanced Raman scattering (SERS) spectra of tryptophan (Trp) were obtained. A unique SERS spectrum of Trp, corresponding to the most stable conformation and orientation on the metal surface, is observed after a ...
Inducción de la enzima triptófano 2,3 dioxigenasa por glucocorticoides y su papel en la tolerancia materna al feto
(Universidad de Antioquia, Facultad de MedicinaBiogénesisGrupo ReproducciónMedellín, Colombia, 2022)
The peroxyl radical-induced oxidation of Escherichia coli FtsZ and its single tryptophan mutant (Y222W) modifies specific side-chains, generates protein cross-links and affects biological function
(Elsevier Inc., 2017)
© 2017 Elsevier Inc. FtsZ (filamenting temperature-sensitive mutant Z) is a key protein in bacteria cell division. The wild-type Escherichia coli FtsZ sequence (FtsZwt) contains three tyrosine (Tyr, Y) and sixteen methionine ...
Comparative steady-state fluorescence studies of cytosolic rat liver (GTP), Saccharomyces cerevisiae (ATP) and Escherichia coli (ATP) phosphoenolpyruvate carboxykinases
(1993)
Two members of the ATP-dependent class of phosphoenolpyruvate (PEP) carboxykinases (Saccharomyces cerevisiae and Escherichia coli PEP carboxykinase), and one member of the GTP-dependent class (the cytosolic rat liver enzyme) ...
Impact of bacterial metabolites on gut barrier function and host immunity: a focus on bacterial metabolism and its relevance for intestinal inflammation
(Frontiers Media, 2021)
The diverse and dynamic microbial community of the human gastrointestinal tract plays a vital role in health, with gut microbiota supporting the development and function of the gut immune barrier. Crosstalk between ...
Riboflavin-photosensitized anaerobic modification of rat lens proteins. A correlation with age-related changes
(1992)
When rat lens homogenate or its soluble protein fractions are irradiated in the presence of riboflavin, a photo-adduct is obtained between this vitamin and the lens proteins. Irradiation of these proteins in the presence ...
Differences in nucleotide-binding site of isoapyrases deduced from tryptophan fluorescence
(PERGAMON-ELSEVIER SCIENCE LTD, 2003-05)
Comparative studies of intrinsic and extrinsic fluorescence of apyrases purified from two potato tuber varieties (Pimpernel and Desiree) were performed to determine differences in the microenvironment of the nucleotide ...
Domain folding and flexibility of Escherichia coli FtsZ determined by tryptophan site-directed mutagenesis
(COLD SPRING HARBOR LAB PRESS, 2007-08)
FtsZ has two domains, the amino GTPase domain with a Rossmann fold, and the carboxyl domain that
resembles the chorismate mutase fold. Bioinformatics analyses suggest that the interdomain interaction
is stronger than the ...
Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant Saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography
(SPRINGER, 2008-08)
During cationic bed adsorption (EBA), with cutinase with varying length tryptophan tags (WP)(2) and (WP)(4), 33% and 10% of adsorption capacity and 80% and 32% eluted specific activity were observed in relation to wild ...