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Free energy contributions to direct readout of a DNA sequence
(American Society for Biochemistry and Molecular Biology, 2005-09)
The energetic contributions of individual DNA-contacting side chains to specific DNA recognition in the human papillomavirus 16 E2C-DNA complex is small (less than 1.0 kcal mol-1), independent of the physical and chemical ...
Human DNA degradation assessment and male DNA detection by quantitative-PCR followed by high-resolution melting analysis
(Elsevier Ireland, 2019-02)
We developed a q-PCR technique that simultaneously evaluates the extent of degradation and determines the gender of a human DNA donor. QYDEG HRM is a triplex real-time PCR whose products are analysed by high-resolution ...
Direct DNA amplification from Fall Armyworm (Lepidoptera: Noctuidae) samples
(Florida Entomological Soc, 2013-11)
The direct amplification by PCR of the DNA in tissue samples of eggs and neonate larvae of Spodoptera frugip erda (J. E. Smith) (Lepidoptera: Noctuidae) was accomplished, and it is a new alternative for DNA amplification ...
Unsupervised fuzzy binning of metagenomic sequence fragments on three-dimensional Barnes-Hut t-Stochastic Neighbor Embeddings
(Institute of Electrical and Electronics Engineers Inc., 2018-01-01)
Shotgun metagenomic studies attempt to reconstruct population genome sequences from complex microbial communities. In some traditional genome demarcation approaches, high-dimensional sequence data are embedded into ...
Environmental DNA metabarcoding: an approach for biodiversity monitoringMetabarcoding de DNA ambiental: un enfoque para el seguimiento de la biodiversidad
(Universidad Autónoma de Tamaulipas, 2021)
Generation of sequence-specific, high affinity anti-DNA antibodies.
(American Society for Biochemistry and Molecular Biology, 2001-12)
By taking advantage of the extreme stability of a protein-DNA complex, we have obtained two highly specific monoclonal antibodies against a predetermined palindromic DNA sequence corresponding to the binding site of the ...
Rapid mitochondrial genome sequencing based on Oxford Nanopore Sequencing and a proxy for vertebrate species identification
(John Wiley and Sons Ltd, 2020-01-01)
Molecular information is crucial for species identification when facing challenging morphology-based specimen identifications. The use of DNA barcodes partially solves this problem, but in some cases when PCR is not an ...