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Novel intein-based self-cleaving affinity tag for recombinant protein production in Escherichia coli
(Elsevier Science, 2021-05)
We evaluated several intein-based self-cleaving affinity tags for expression and single-step affinity chromatography purification of recombinant proteins produced in Escherichia coli. We used human growth hormone (hGH) as ...
A simplified strategy to package foreign proteins into baculovirus occlusion bodies without engineering the viral genome
(Elsevier Science, 2020-01-10)
Polyhedron envelope protein (PEP) is the major component of the calyx that surrounds the baculovirus occlusion body (OB). PEP has been associated with the stabilization and resistance of polyhedra in the environment. Due ...
GFP transient expression and silencing in fragaria x ananassa
(IOS Press, 2020-01)
Background: Stable transformation, transient expression, and post-transcriptional gene silencing (PTGS) are powerful methodologies that allow exploration of gene function. Objective: We aimed to apply these methodologies ...
High hydrostatic pressure and soybean protein addition to beef patties: Effects on the formation of mixed aggregates and technological parameters
(Elsevier, 2020-12)
The effect of replacing 20% of meat proteins with soy protein isolate (SPI) and high hydrostatic pressure (HHP, 300 MPa-5 min) on technological, color and texture parameters of beef patties was studied. Also, HHP-induced ...
Alteration in localization of steroid hormone receptors and coregulatory proteins in follicles from cows with induced ovarian follicular cysts
(BioScientifica, 2012-12)
Cystic ovarian disease (COD) is an important cause of infertility in cattle. The altered follicular dynamics and cellular differentiation observed in COD may be mediated through a disruption of the expression of steroid ...
Overexpression of survivin in pediatric Hodgkin lymphoma tumor cells: Characterization of protein expression and splice-variants transcription profile
(Academic Press Inc Elsevier Science, 2019-06)
Survivin is abundantly expressed during fetal development but absent in most differentiated adult tissues; an exception being components of the immune system, such as B and T lymphocytes. Beyond acting as a master regulator ...
Reverse Engineering of an Aspirin-Responsive Transcriptional Regulator in Escherichia coli
(American Chemical Society, 2019-07)
Bacterial transcription factors (TFs) are key devices for the engineering of complex circuits in many biotechnological applications, yet there are few well-characterized inducer-responsive TFs that could be used in the ...
Proteomic analysis of Listeria monocytogenes FBUNT during biofilm formation at 10°C in response to Lactocin AL705
(Frontiers Research Foundation, 2021-01-29)
Listeria monocytogenes is one of the major food-related pathogens and is able to survive and multiply under different stress conditions. Its persistence in industrial premises and foods is partially due to its ability to ...