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Novel intein-based self-cleaving affinity tag for recombinant protein production in Escherichia coli
(Elsevier Science, 2021-05)
We evaluated several intein-based self-cleaving affinity tags for expression and single-step affinity chromatography purification of recombinant proteins produced in Escherichia coli. We used human growth hormone (hGH) as ...
Overexpressed Proteins in Hypervirulent Clade 8 and Clade 6 Strains of Escherichia coli O157:H7 Compared to E. coli O157:H7 EDL933 Clade 3 Strain
(Public Library of Science, 2016-11)
Escherichia coli O157:H7 is responsible for severe diarrhea and hemolytic uremic syndrome (HUS), and predominantly affects children under 5 years. The major virulence traits are Shiga toxins, necessary to develop HUS and ...
Early Events of Enterotoxigenic Escherichia coli Colonization on Gut Barrier Function: No Longer UndETECted?
(Springer, 2017-04)
Enterotoxigenic Escherichia coli (ETEC) strains are involved in piglet post-weaning diarrhea. The early effects of ETEC infection can be modulated by probiotics added to feed like Enterococcus faecium. TEC affect intestinal ...
Small qnrB-harbouring ColE-like plasmids widespread in commensal enterobacteria from a remote Amazonas population not exposed to antibiotics
(Oxford University Press, 2011)
Sir, After a decade since discovery, a variety of qnr-type plasmid-mediated quinolone resistance genes have been reported worldwide, especially in clinical isolates of Enterobacteriaceae.1 Recently, we found that qnr genes ...
Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain
(2016)
Escherichia coli O157:H7 is responsible for severe diarrhea and hemolytic uremic syndrome (HUS), and predominantly affects children under 5 years. The major virulence traits are Shiga toxins, necessary to develop HUS and ...
In vivo bactericidal efficacy of gwh1 antimicrobial peptide displayed on protein nanoparticles, a potential alternative to antibiotics
(MDPI AG, 2020-12)
Oligomerization of antimicrobial peptides into nanosized supramolecular complexes produced in biological systems (inclusion bodies and self-assembling nanoparticles) seems an appealing alternative to conventional antibiotics. ...
Application of response surface methodology and artificial neural networks for optimization of recombinant Oryza sativa non-symbiotic hemoglobin 1 production by escherichia coli in medium containing byproduct glycerol
(Elsevier, 2010-10)
Production of recombinant Oryza sativa non-symbiotic hemoglobin 1 (OsHb1) by Escherichia coli was maximized in shake-flask cultures in media containing tryptone, yeast extract, sodium chloride and byproduct glycerol from ...
Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli
(Elsevier Science, 2019-10)
Active packaging materials with antimicrobial properties have gained significant interest over the last decade. Furthermore, phages are potentially useful as antimicrobial agents in food. Phage stability assays were conducted ...
Expression of M Protein from LP02/C Equine Arteritis Virus Inhibits Growth of Escherichia Coli M15-pQE30 System
(Open Acces text, 2017-05)
Our objective was to obtain Equine Arteritis Virus M protein in prokaryotic system to test it as immunogen. LP02/C Equine Arteritis Virus cDNA was used astemplate to obtain and clone this protein. Equine Arteritis M protein ...
In vitro development and analysis of escherichia coli and shigella boydii azithromycin-resistant mutants
(Mary Ann Liebert, 2013)
Three clinical isolates of E. coli and one S. boydii isolated from feces samples collected from children under 5 years of age with diarrhea in Lima, Peru were inoculated onto Mueller-Hinton plates containing increasing ...