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Correlation between catalysis and tertiary structure arrangement in an archaeal halophilic subtilase
(Elsevier Masson, 2012-03)
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon N. magadii that exhibits optimal activity and stability in salt-saturated solutions. In this work, the ...
Activity and stability of lipase preparations from Penicillium corylophilum: Potential use in biocatalysis
(Wiley-VCH, 2014-11)
Biocatalysts with lipase activity were produced by Penicillium corylophilum under basal and olive oil-induced conditions. In the presence of olive oil, both the extracellular and the mycelium-bound lipase activities were ...
Heterologous production and characterization of a thermostable GH10 family endo-xylanase from Pycnoporus sanguineus BAFC 2126
(Elsevier, 2018-04)
Xylanases are key enzymes for agricultural biomass saccharification for the production of cellulosic ethanol. Success in enzymatic lignocellulose bioconversion is restricted by enzyme production costs, activity and stability ...
Membrane anchoring stabilizes and favors secretion of New Delhi metallo-β-lactamase
(Nature Publishing Group, 2016-07)
Carbapenems, 'last-resort' β-lactam antibiotics, are inactivated by zinc-dependent metallo-β-lactamases (MBLs). The host innate immune response withholds nutrient metal ions from microbial pathogens by releasing metal-chelating ...
Assessment of the effect of silicon on antioxidant enzymes in cotton plants by multivariate analysis
(American Chemical Society, 2013-11)
Silicon has been extensively researched in relation to the response of plants to biotic and abiotic stress, as an element triggering defense mechanisms which activate the antioxidant system. Furthermore, in some species, ...
Characterization of poly(ADP-ribose)polymerase from Crithidia fasciculata: Enzyme inhibition by β-lapachone
(Elsevier Science, 2001-12)
Crithidia fasciculata poly(ADP-ribose)polymerase (PARP) has been isolated and partially purified. This is the first PARP isolated from trypanosomatids; it requires DNA and histone for activity, using NAD+ as substrate. ...
The ubiquitin-conjugating enzyme CDC34 is essential for cytokinesis in contrast to putative subunits of a SCF complex in Trypanosoma brucei
(Public Library of Science, 2017-06-13)
The ubiquitin-proteasome system is a post-translational regulatory pathway for controlling protein stability and activity that underlies many fundamental cellular processes, including cell cycle progression. Target proteins ...
Purification and partial characterization of an acidic polygalacturonase from Aspergillus kawachii
(Elsevier Science, 2004-05)
An endo-polygalacturonase, named PGI, was purified to homogeneity from the culture filtrate of Aspergillus kawachii IFO 4033 grown in a glucose-tryptone medium. The molecular mass of PGI was estimated to be 60kDa by SDS-PAGE ...
Improving the properties of chitosan as support for the covalent multipoint immobilization of chymotrypsin
(American Chemical Society, 2008-07)
Changing gel structure and immobilization conditions led to a significant improvement in the covalent multipoint attachment of chymotrypsin on chitosan. The use of sodium alginate, gelatin, or κ-carrageenan, activation ...
Reversible Unfolding of a Thermophilic Membrane Protein in Phospholipid/Detergent Mixed Micelles
(Academic Press Ltd - Elsevier Science Ltd, 2010-03)
Folding mechanisms and stability of membrane proteins are poorly understood because of the known difficulties in finding experimental conditions under which reversible denaturation could be possible. In this work, we ...