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A polymerase chain reaction for detection of equine herpesvirus-1 in routine diagnostic submissions of tissues from aborted foetuses
(Wiley, 2001-05)
Equine herpesvirus 1 (EHV-1) is the causative agent of abortion, perinatal foal mortality, neurological and acute respiratory diseases in horses. Conventional laboratory diagnosis involving viral isolation from aborted ...
Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)
(Elsevier Science, 2011-04-19)
Paramphistomosis has shown an increased spread over the last years in Argentina, being in some regions an emerging parasitosis, which has motivated their study and identification. Even when morphological features were ...
Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing
(John Wiley & Sons Inc, 2019-08)
The clustered regularly interspaced short palindromic repeats (CRISPR) system?associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored ...
Mus81-Eme1-dependent aberrant processing of DNA replication intermediates in mitosis impairs genome integrity
(Science Advances is the American Association for the Advancement of Science, 2020-12)
Chromosome instability (CIN) underpins cancer evolution and is associated with drug resistance and poor prognosis. Understanding the mechanistic basis of CIN is thus a priority. The structure-specific endonuclease Mus81-Eme1 ...
SNP genotyping by heteroduplex analysis
(Springer, 2015-10)
Heteroduplex-based genotyping methods have proven to be technologically effective and economically efficient for low- to medium-range throughput single-nucleotide polymorphism (SNP) determination. In this chapter we describe ...
A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
(Sage Publications, 2020-02)
Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization ...
Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
(Elsevier, 2012-04)
Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction–restriction fragment length polymorphism ...
Development of a Recombination System for the Generation of Occlusion Positive Genetically Modified Anticarsia Gemmatalis Multiple Nucleopolyhedrovirus
(2015)
Anticarsia gemmatalis is an important pest in legume crops in South America and it has been successfully controlled using Anticarsia gemmatalis Multiple Nucleopolyhedrovirus(AgMNPV) in subtropical climate zones. Nevertheless, ...
RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
(Oxford University Press, 2014-06)
For toxin/antitoxin (TA) systems, no toxin has been identified that functions by cleaving DNA. Here, we demonstrate that RalR and RalA of the cryptic prophage rac form a type I TA pair in which the antitoxin RNA is a ...
Occurrence of bovine herpesvirus type 4 DNA in Argentinean Holstein cattle from Santiago del Estero, ArgentinaOcorrência de DNA de herpesvírus bovino tipo 4 em gado Holstein argentino de Santiago del Estero, Argentina
(Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia, 2011-12)
Bovine herpesvirus type 4 (BoHV-4) is a gamma-herpesvirus that has been isolated from apparently asymptomatic animals and from cattle with a variety of clinical signs. The pathogenic role of BoHV-4 remains unclear and it ...