Synaptophysin is a selective marker for axons in human cutaneous end organ complexes

dc.creatorGarcía-Mesa, Yolanda
dc.creatorGarcía-Piqueras, Jorge
dc.creatorCuendias, Patricia
dc.creatorCobo, Ramón
dc.creatorMartín-Cruces, José
dc.creatorFeito, Jorge
dc.creatorGarcía-Suarez, Olivia
dc.creatorBiedma, Benjamín Martín
dc.creatorVega, J.A.
dc.date2024-04-10T05:55:54Z
dc.date2024-04-10T05:55:54Z
dc.date2022
dc.date.accessioned2024-07-17T21:12:42Z
dc.date.available2024-07-17T21:12:42Z
dc.identifier10.1016/j.aanat.2022.151955
dc.identifier09409602
dc.identifierhttps://hdl.handle.net/20.500.12728/10857
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9509351
dc.descriptionBackground: Small clear synaptic-like vesicles fill axon terminals of mechanoreceptors. Their functional significance is controversial and probably includes release of neurotransmitters from afferent axon terminals. Synaptophysin, a major protein of the synaptic vesicle membrane, is present in presynaptic endings of the central and peripheral nervous systems. It is also expressed in mechanosensory neurons which extend into skin forming sensory corpuscles. Nevertheless, synaptophysin occurrence in these structures has never been investigated. Methods: Here we used immunohistochemistry to detect synaptophysin in adult human dorsal root ganglia, cutaneous Meissner and Pacinian corpuscles and Merkel cell-neurite complexes from foetal to elderly period. Moreover, we analyzed whether synaptophysin co-localizes with the mechano-gated protein PIEZO2. Results: Synaptophysin immunoreactivity was observed in primary sensory neurons (36 ± 6%) covering the entire soma size ranges. Axons of Meissner's and Pacinian corpuscles were positive for synaptophysin from 36 and 12 weeks of estimated gestational age respectively, to 72 years old. Synaptophysin was also detected in Merkel cells (from 14 weeks of estimated gestational age to old age). Additionally in adult skin, synaptophysin and PIEZO2 co-localized in the axon of Meissner and Pacinian corpuscles, Merkel cells as well as in some axons of Merkel cell-neurite complexes. Conclusion: Present results demonstrate that a subpopulation of primary sensory neurons and their axon terminals forming cutaneous sensory corpuscles contain synaptophysin, a typical presynaptic vesicle protein. Although the functional relevance of these findings is unknown it might be related to neurotransmission mechanisms linked to mechanotransduction. © 2022 The Author(s)
dc.descriptionGerencia Regional de Salud de Castilla y León, (GRS 1882/A/18, PA-18-PF-BP17-044, PA-21-PF-BP20-122); Gobierno del Principado de Asturias, GPA
dc.formatapplication/pdf
dc.languageen
dc.publisherElsevier GmbH
dc.subjectHuman
dc.subjectImmunohistochemistry
dc.subjectMeisner corpuscles
dc.subjectMerkel cell- neurite complexes
dc.subjectPacinian corpuscles
dc.subjectPIEZO2
dc.subjectSynaptophysin
dc.titleSynaptophysin is a selective marker for axons in human cutaneous end organ complexes
dc.typeArticle


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