info:eu-repo/semantics/article
Lineage II of Southeast Asian/American DENV-2 is Associated with a Severe Dengue Outbreak in the Peruvian Amazon
Fecha
2014-07-07Registro en:
Lineage II of Southeast Asian/American DENV-2 is Associated with a Severe Dengue Outbreak in the Peruvian Amazon 2014, 91 (3):611 American Journal of Tropical Medicine and Hygiene
0002-9637
10.4269/ajtmh.13-0600
American Journal of Tropical Medicine and Hygiene (Am. J. Trop. Med. Hyg)
Autor
Williams, M.
Mayer, S. V.
Johnson, W. L.
Chen, R.
Volkova, E.
Vilcarromero, S.
Widen, S. G.
Wood, T. G.
Suárez Ognio, Luis
Long, K. C.
Hanley, K. A.
Morrison, A. C.
Vasilakis, N.
Halsey, E. S.
Institución
Resumen
During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak.