Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

dc.creatorAguirre, Carlos
dc.creatorOlivares, Natalia
dc.creatorHinrichsen, Patricio
dc.creatorSanchez, Evelyn [Univ Mayor, Fac Ciencias, Ctr Genom & Bioinformat, Chile]
dc.date.accessioned2023-11-28T16:55:33Z
dc.date.accessioned2024-05-02T20:49:57Z
dc.date.available2023-11-28T16:55:33Z
dc.date.available2024-05-02T20:49:57Z
dc.date.created2023-11-28T16:55:33Z
dc.date.issued2021-06-04
dc.identifierAguirre, C., Sánchez, E., Olivares, N., & Hinrichsen, P. (2021). Multiplex TaqMan real-time PCR assay for sensitive detection of two weevil species (Coleoptera: Curculionidae). Journal of Economic Entomology, 114(1), 90-99.
dc.identifier0022-0493
dc.identifiereISSN: 2309-5807
dc.identifierWOS: 000648959000010
dc.identifierPMID: 33155656
dc.identifierhttps://repositorio.umayor.cl/xmlui/handle/sibum/9048
dc.identifierhttps://doi.org/10.1093/jee/toaa251
dc.identifierhttps://academic.oup.com/jee/article-pdf/114/1/90/36217077/toaa251.pdf
dc.identifierhttps://watermark.silverchair.com/toaa251.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA18wggNbBgkqhkiG9w0BBwagggNMMIIDSAIBADCCA0EGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMmg5Xol_l7494cNhsAgEQgIIDEu--tiA04Mn7iaiVTwqKfMTWH1f4KumN_kkhqO6A2_y1nbdOLlstu-zFlx83Mn-cJaa8dmAaX3jQiB9-OhCHrb3jp4HTIQVz4T2-Ks8nP5rrocEB7CJdIY1MYGu8DtfAPRltmQ8yU3JenzrblHvbGsKhUgKVq6gzlKyEwrDMgz8eV1pROeuLbGGvkJWnJ1vLIQIRap4keOl1DTBkf7rv1GDZUP7QKmPWml8aMlDwSfKHksdaQXHBdnu8pJu_sh8c-vA2e7i_somU2lEQfUu0o2dzogHG5vx_s-lYcZOQ_-iPQYRYRTmOa6BhiFyXzdY_-ONQkHfIyXFFRTbFU9eOExDa_lHUYbAslWQPd4FPz4hKmYHfhTNYW4njjJRwPi6FTp7RvXy7iajWZ3Bx1BjxAmh8-Tr5kHYl6wLchT1Svj0pZMEtA0YCEbqCBPk-GNxgwJPy0iE5kv5VkpwU5Es7K5CJmQ7SxuVe6yGd2_RscOXz9RMZHoiv7UDUMLQVMtHglusiFI_TJ0P9kVdVHdJS0aaNo9yI_faT07d74iBN05fcKYprhj3MRy5gVcQdZUZPnTkqzIPA1rz9RIhW147uHrmhA3-S1feroBRN1ZpFaft1owVpvQWWvC1t3wofqaG4qKRIhaSELjnyNfaI3b1msVZtprX_E1F5Y2BeRxgOB5yhnHsx9rPB4diG8QW7TzxZU9zkmgrjryPyggusluwFz-R58Pr7yq14KCIEty-mJL8pfJezX7YJLbPVuTxOU9n6Fk3xTQ7SwNNSVReux9heILnjCjgKywouBdzMtmzbB9bQyMDgn8-DjR3qFYdJRSOLaHQvQZOctm_OB-GupcL3uVhbAhSeFWbfe-dUdSjUF2IaB9J8QwjuyVDiLNQth269-uWqG7xg4Iv2kJWdGIeAyusvs1Qt1cm0DcrH_cff-D-3IZqJ4R4s7_kzB81JpS4M3-DGhI8z3hcf3TqmTxt2v5sJD2rxfUUoglGJcCccgNgLSTLNiUfd0rxOyS0vBplQnTEu3q0kY2_NKRQGjaAG1THp6g
dc.identifier10.1093/jee/toaa251
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9275834
dc.description.abstractRapid and cost-efficient identification of Naupactus species is becoming a key process for the exportation of citrus fruit from Chile and other countries, considering the quarantine regulations for some species of the cosmopolitan genus Naupactus. This study deals with the development of a fast and sensitive detection protocol for Naupactus cervinus (Coleoptera: Curculionidae) (Boheman) and Naupactus xanthographus (Coleoptera: Curculionidae) (Germar) based on multiplex TaqMan Real-time polymerase chain reaction. Both N. cervinus and N. xanthographus primer and probe sets achieved species-specific detection in a linear range from 1 pg/mu l to 1 x 10(-6) pg/mu l, allowing detection of as few as 160 copies of template DNA. Non-target amplifications were not detected and a panel composed of 480 test samples had 100% coincidence with the respective morphological identification.
dc.languageen_US
dc.publisherOXFORD UNIV PRESS INC
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.titleMultiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)
dc.typeArtículo o Paper


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