| dc.creator | Flores-Aldama, Lisandra | |
| dc.creator | Bustos, Daniel | |
| dc.creator | Cabezas-Bratesco, Deny | |
| dc.creator | Gonzalez, Wendy | |
| dc.creator | Brauchi, Sebastian E | |
| dc.date | 2023-03-29T12:39:11Z | |
| dc.date | 2023-03-29T12:39:11Z | |
| dc.date | 2023 | |
| dc.date.accessioned | 2024-05-02T20:30:51Z | |
| dc.date.available | 2024-05-02T20:30:51Z | |
| dc.identifier | http://repositorio.ucm.cl/handle/ucm/4599 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/9274839 | |
| dc.description | TRPV5 and TRPV6 are calcium-selective ion channels expressed at the apical membrane of
epithelial cells. Important for systemic calcium (Ca2+) homeostasis, these channels are considered
gatekeepers of this cation transcellular transport. Intracellular Ca2+ exerts a negative control over
the activity of these channels by promoting inactivation. TRPV5 and TRPV6 inactivation has been
divided into fast and slow phases based on their kinetics. While slow inactivation is common to both
channels, fast inactivation is characteristic of TRPV6. It has been proposed that the fast phase depends
on Ca2+ binding and that the slow phase depends on the binding of the Ca2+/Calmodulin complex
to the internal gate of the channels. Here, by means of structural analyses, site-directed mutagenesis,
electrophysiology, and molecular dynamic simulations, we identified a specific set of amino acids and
interactions that determine the inactivation kinetics of mammalian TRPV5 and TRPV6 channels. We
propose that the association between the intracellular helix-loop-helix (HLH) domain and the TRP
domain helix (TDh) favors the faster inactivation kinetics observed in mammalian TRPV6 channels. | |
| dc.language | en | |
| dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
| dc.source | International Journal of Molecular Sciences, 24(5), 4470 | |
| dc.subject | TRP channels | |
| dc.subject | Inactivation | |
| dc.subject | TRPV5 | |
| dc.subject | TRPV6 | |
| dc.subject | Molecular evolution | |
| dc.title | Intracellular Helix-Loop-Helix domain modulates inactivation kinetics of mammalian TRPV5 and TRPV6 channels | |
| dc.type | Article | |
