artículo
Abnormal distribution of inositol 1,4,5-trisphosphate receptors in human muscle can be related to altered calcium signals and gene expression in Duchenne dystrophy-derived cells
Fecha
2010Registro en:
10.1096/fj.09-152017
1530-6860
0892-6638
MEDLINE:20395455
WOS:000281446400010
Autor
Cardenas, Cesar
Juretic, Nevenka
Bevilacqua, Jorge A.
Garcia, Isaac E.
Figueroa, Reinaldo
Hartley, Ricardo
Taratuto, Ana L.
Gejman, Roger
Riveros, Nora
Molgo, Jordi
Jaimovich, Enrique
Institución
Resumen
Inositol 1,4,5-trisphosphate (IP3) receptors (IP(3)Rs) drive calcium signals involved in skeletal muscle excitation-transcription coupling and plasticity; IP3R subtype distribution and downstream events evoked by their activation have not been studied in human muscle nor has their possible alteration in Duchenne muscular dystrophy (DMD). We studied the expression and localization of IP3R subtypes in normal and DMD human muscle and in normal (RCMH) and dystrophic (RCDMD) human muscle cell lines. In normal muscle, both type 1 IP(3)Rs (IP(3)R1) and type 2 IP(3)Rs (IP(3)R2) show a higher expression in type II fibers, whereas type 3 IP(3)Rs (IP(3)R3) show uniform distribution. In DMD biopsies, all fibers display a homogeneous IP(3)R2 label, whereas 24 +/- 7% of type II fibers have lost the IP(3)R1 label. RCDMD cells show 5-fold overexpression of IP(3)R2 and down-regulation of IP(3)R3 compared with RCMH cells. A tetanic stimulus induces IP3-dependent slow Ca2+ transients significantly larger and faster in RCDMD cells than in RCMH cells as well as significant ERK1/2 phosphorylation in normal but not in dystrophic cells. Excitation-driven gene expression was different among cell lines; 44 common genes were repressed in RCMH cells and expressed in RCDMD cells or vice versa. IP3-dependent Ca2+ release may play a significant role in DMD pathophysiology.-Cardenas, C., Juretic, N., Bevilacqua, J. A., Garcia, I. E., Figueroa, R., Hartley, R., Taratuto, A. L., Gejman, R., Riveros, N., Molgo, J., Jaimovich, E. Abnormal distribution of inositol 1,4,5-trisphosphate receptors in human muscle can be related to altered calcium signals and gene expression in Duchenne dystrophy-derived cells. FASEB J. 24, 3210-3221 (2010). www.fasebj.org
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Relationship between Skin Temperature, Electrical Manifestations of Muscle Fatigue, and Exercise-Induced Delayed Onset Muscle Soreness for Dynamic Contractions : A Preliminary Study
Priego Quesada, J. I.; De la Fuente, Carlos; Kunzler, M. R.; Pérez Soriano, P.; Hervás Marín, D.; Carpes, Felipe P. (2020) -
Andrographolide attenuates skeletal muscle dystrophy in mdx mice and increases efficiency of cell therapy by reducing fibrosis
Cabrera, Daniel; Gutierrez, Jaime; Cabello-Verrugio, Claudio; Gabriela Morales, Maria; Mezzano, Sergio; Fadic, Ricardo; Carlos Casar, Juan; Hancke, Juan L.; Brandan, Enrique -
Glypican-1 regulates myoblast response to hgf via met in a lipid raft-dependent mechanism: effect on migration of skeletal muscle precursor cells
Gutierrez, Jaime; Cabrera, Daniel; Brandan, Enrique