info:eu-repo/semantics/article
Duplication and Diversification of REPLUMLESS – A Case Study in the Papaveraceae
Registro en:
Zumajo-Cardona C, Pabón-Mora N, Ambrose BA. Duplication and Diversification of REPLUMLESS - A Case Study in the Papaveraceae. Front Plant Sci. 2018 Dec 12;9:1833. doi: 10.3389/fpls.2018.01833.
1664-462X
10.3389/fpls.2018.01833
Autor
Pabón Mora, Natalia
Zumajo Cardona, Cecilia
Ambrose, Barbara A.
Institución
Resumen
ABSTRACT: There is a vast amount of fruit morphological diversity in terms of their texture, the
number of carpels, if those carpels are fused or not and how fruits open to disperse
the seeds. Arabidopsis thaliana, a model eudicot, has a dry bicarpellate silique, when
the fruit matures, the two valves fall apart through the dehiscence zone leaving the seeds
attached to the remaining medial tissue, called the replum. Proper replum development
in A. thaliana is mediated by REPLUMLESS (RPL), a TALE Homeodomain protein. RPL
represses the valve margin genetic program and the downstream dehiscence zone
formation in the medial tissue of the siliques and RPL orthologs have conserved roles
across the Brassicaceae eudicots. A RPL homolog, qSH1, has been studied in rice,
a monocot, and plays a role in fruit shedding making it difficult to predict functional
evolution of this gene lineage across angiosperms. Although RPL orthologs have been
identified across all angiosperms, expression and functional analyses are scarce. In
order to fill the phylogenetic gap between the Brassicaceae and monocots we have
characterized the expression patterns of RPL homologs in two poppies with different
fruit types, Bocconia frutescens with operculate valvate dehiscence and a persistent
medial tissue, similar to a replum, and Papaver somniferum, a poppy with persistent
medial tissue in between the multicarpellate gynoecia. We found that RPL homologs in
Papaveraceae have broad expression patterns during plant development; in the shoot
apical meristem, during flowering transition and in many floral organs, especially the
carpels. These patterns are similar to those of RPL in A. thaliana. However, our results
suggest that RPL does not have conserved roles in the maintenance of medial persistent
tissues of fruits but may be involved with establishing the putative dehiscence zone in
dry poppy fruits. COL0170292