Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1

dc.creatorCândido, Alexandre Luna
dc.creatorBontempo, Elizabete Dias
dc.creatorResende, Maurício
dc.date2014-02-03T19:22:58Z
dc.date2014-02-03T19:22:58Z
dc.date2010-04
dc.date.accessioned2023-09-28T22:45:53Z
dc.date.available2023-09-28T22:45:53Z
dc.identifierCANDIDO, A. L.; BONTEMPO, E. D.; RESENDE, M. Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 2, abr. 2000. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015&lng=en&nrm=iso>. Acesso em: 3 fev. 2014.
dc.identifier0074-0276
dc.identifierhttps://ri.ufs.br/handle/riufs/865
dc.identifierCreative Commons Attribution License
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/9077353
dc.descriptionEspecial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.
dc.formatapplication/pdf
dc.languageen
dc.publisherFundação Oswaldo Cruz
dc.subjectHerpesvirus bovino
dc.subjectCo-solventes
dc.subjectDNA
dc.subjectReação em cadeia da polimerase
dc.titlePolymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
dc.typeArtigo


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