Artigo
Evaluation of the infection process by Lecanicillium fungicola in Agaricus bisporus by scanning electron microscopy
Evaluación del proceso de infección por Lecanicillium fungicola en Agaricus bisporus por microscopia electrónica de barrido
Registro en:
NUNES, J. S. et al. Evaluation of the infection process by Lecanicillium fungicola in Agaricus bisporus by scanning electron microscopy. Revista Iberoamericana de Micología, Barcelona, v. 34, n. 1, p. 36-42, Jan./Mar. 2017.
Autor
Nunes, Janaira Santana
Brito, Manuela Rocha de
Zied, Diego Cunha
Leite, Eloisa Aparecida das Graças
Dias, Eustáquio Souza
Alves, Eduardo
Institución
Resumen
Background: Lecanicillium fungicola causes dry bubble disease in Agaricus bisporus mushrooms leading to
significant economic losses in commercial production.
Aims: To monitor the infection process of L. fungicola in Brazilian strains of A. bisporus.
Methods: The interaction between the mycelium of L. fungicola (LF.1) and three strains of A. bisporus
(ABI 7, ABI 11/14 and ABI 11/21) was studied. Electron microscopy and X-ray microanalyses of vegetative
growth and basidiocarp infection were evaluated.
Results: Micrographs show that the vegetative mycelium of the Brazilian strains of A. bisporus is not
infected by the parasite. The images show that the pathogen can interlace the hyphae of A. bisporus without
causing damage, which contributes to the presence of L. fungicola during the substrate colonization,
allowing their presence during primordial formation of A. bisporus. In the basidiocarp, germ tubes form
within 16 h of infection with L. fungicola and the beginning of penetration takes place within 18 h, both
without the formation of specialized structures.
Conclusions: Scanning electron microscopy enabled the process of colonization and reproduction to be
observed within the formation of phialides, conidiophores and verticils of L. fungicola. The formation
of calcium oxalate crystals by the pathogen was also visible using the X-ray microanalysis, both at the
hyphae in the Petri plate and at basidiocarp infection site