Artigo
Hypothalamic stearoyl-CoA desaturase-2 (SCD2) controls whole-body energy expenditure
Registro en:
MOURA, R. F. de et al. Hypothalamic stearoyl-CoA desaturase-2 (SCD2) controls whole-body energy expenditure. International Journal of Obesity, London, v. 40, p. 471-478, 2016.
Autor
Moura, Rodrigo F. de
Nascimento, Lucas F.
Ignacio-Souza, Leticia M.
Morari, Joseane
Razolli, Daniela S.
Solon, Carina
Souza, Gabriela F. P. de
Festuccia, William T.
Velloso, Licio A.
Institución
Resumen
Background/Objectives:
Stearoyl-CoA desaturase-2 (SCD2) is the main δ9 desaturase expressed in the central nervous system. Because of its potential involvement in controlling whole-body adiposity, we evaluated the expression and function of SCD2 in the hypothalami of mice.
Subjects/Methods:
Male mice of different strains were used in real-time PCR, immunoblot and metabolic experiments. In addition, antisense oligonucleotides and lentiviral vectors were used to reduce and increase the expression of SCD2 in the hypothalamus.
Results:
The level of SCD2 in the hypothalamus is similar to other regions of the central nervous system and is ~10-fold higher than in any other region of the body. In the arcuate nucleus, SCD2 is expressed in proopiomelanocortin and neuropeptide-Y neurons. Upon high fat feeding, the level of hypothalamic SCD2 increases. Inhibition of hypothalamic SCD2 as accomplished by two distinct approaches, an antisense oligonucleotide or a short-hairpin RNA delivered by a lentivirus, resulted in reduced body mass gain mostly due to increased energy expenditure and increased spontaneous activity. Increasing hypothalamic SCD2 by a lentivirus approach resulted in no change in body mass and food intake.
Conclusions:
Thus, SCD2 is highly expressed in the hypothalami of rodents and its knockdown reduces body mass due to increased whole-body energy expenditure.