Texto completo de evento
In vitro effect of phototherapy with low intensity laser on HSV-1 and epithelial cells
Registro en:
10.1117/12.700682
0000-0001-7404-9606
Autor
EDUARDO, FERNANDA P.
MEHNERT, DOLORES U.
MONEZI, TELMA A.
ZEZELL, DENISE M.
SCHUBERT, MARK M.
EDUARDO, CARLOS P.
MARQUES, MARCIA M.
SPIE BIOS
Resumen
The effects of phototherapy on herpes lesions have been clinically demonstrated by either preventing the lesion
formation or speeding their repair. The aim of this in vitro study was analyze the effect of phototherapy on epithelial
cells and HSV-1 in culture. Cultures of HSV-1 and epithelial cells (Vero cell line) were used. The irradiations were done
using a GaAlAs laser (660 e 780 nm, 4.0 mm2). One, two and three irradiations with 6 h-intervals were done. The
experimental groups were: Control: non-irradiated; 660 nm and 3 J/cm2 (2.8 sec); 660 nm and 5 J/cm2 (3.8 sec); 780 nm
and 3 J/cm2 (1.9 sec), and 780 nm and 5 J/cm2 (2.5 sec). The HSV-1 cytopatic effect and the cell viability of irradiated
cultures and controls were analyzed in four different conditions: irradiation of non-infected epithelial cells; epithelial
cells irradiated prior infection; virus irradiated prior infection; irradiation of HSV infected cells. The mitochondrial
activity and cytopathic effects were assessed. The number of irradiations influenced the cell growth positively and
proportionally, except for the 660 nm/ 3 J/cm2 group. Any variation in cytopathic effects was observed amongst the
experimental groups. The viability of infected cells prior irradiation was significantly higher than that of non-irradiated
cultures when 2 irradiations were done. Under the experimental conditions of this study we concluded that phototherapy
is capable of enhancing epithelial cell growth and prolonging cell viability of HSV-1 infected cells. Positive benefits of
phototherapy could be resultant from prolongation of infected cells viability, corroborating with host defenses.