Artigo de peri??dico
Monitoring glucose levels in urine using FTIR spectroscopy combined with univariate and multivariate statistical methods
Registro en:
1386-1425
290
10.1016/j.saa.2022.122259
0000-0001-7404-9606
89
85.75
Autor
CAIXETA, DOUGLAS C.
LIMA, CASSIO
XU, YUN
GUEVARA-VEGA, MARCO
ESPINDOLA, FOUED S.
GOODACRE, ROYSTON
ZEZELL, DENISE M.
SABINO-SILVA, ROBINSON
Resumen
The development of novel platforms for non-invasive continuous glucose monitoring applied in the screening and monitoring of diabetes is crucial to improve diabetes surveillance systems. Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy of urine can be an alternative as a sustainable, label-free, fast, non-invasive, and highly sensitive analysis to detect changes in urine promoted by diabetes and insulin treatment. In this study, we used ATR-FTIR to evaluate the urinary components of non-diabetic (ND), diabetic (D), and diabetic insulin-treated (D + I) rats. As expected, insulin treatment was capable to revert changes in glycemia, 24-h urine collection volume, urine creatinine, urea, and glucose excretion promoted by diabetes. Several differences in the urine spectra of ND, D, and D + I were observed, with urea, creatinine, and glucose analytes being related to these changes. Principal components analysis (PCA) scores plots allowed for the discrimination of ND and D + I from D with an accuracy of ??? 99 %. The PCA loadings associated with PC1 confirmed the importance of urea and glucose vibrational modes for this discrimination. Univariate analysis of second derivative spectra showed a high correlation (r: 0.865, p < 0.0001) between the height of 1074 cm-1 vibrational mode with urinary glucose concentration. In order to estimate the amount of glucose present in the infrared spectra from urine, multivariate curve resolution-alternating least square (MCR-ALS) was applied and a higher predicted concentration of glucose in the urine was observed with a correlation of 78.9 % compared to urinary glucose concentration assessed using enzyme assays. In summary, ATR-FTIR combined with univariate and multivariate chemometric analyses provides an innovative, non-invasive, and sustainable approach to diabetes surveillance. Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior (CAPES) Conselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico (CNPq) Funda????o de Amparo ?? Pesquisa do Estado de Minas Gerais (FAPEMIG) CAPES: 88887.506792/2020-00 CNPq: INCT 465763/2014-6; 465669/2014-0; 403193/2022-2; 141629/2015-0 FAPEMIG: APQ-02872-16; APQ-02148-21