Several authors analyzed laser irradiation of dental enamel in an attempt to increase acid resistance and achieve caries prevention. In some cases, the procedure has also been associated to the application of a layer of fluoride. Micro-hardness measurements are reliable tests used to determine the degree of resistance to demineralization. In this work micro-hardness values of superficial dental enamel have been measured before and after irradiation with a 960 nm diode laser, using tested laser parameters that are safe and cause no injuries to the adjacent tissue. Laser parameters were below the dental enamel ablation threshold. 60 enamel samples were used and divided into control group (GC), fluoride application (GF), laser irradiation (GL), fluoride application with subsequent laser irradiation (GFL) and laser with subsequent fluoride application (GLF). A solution containing coal was used on the enamel surface in order to absorb the laser radiation. A statistically significant loss of the micro-hardness value was obtained for all groups except for GL but also this group did not produce significant micro-hardness increase. Conclusion: at the current peak power and beam quality of standard diode laser bars, safe laser parameters do not produce higher micro-hardness values.