Artigo de peri??dico
Optical coherence tomography and polarized light microscopy for the evaluation of artificial caries
Registro en:
0363-6771
1
67
0000-0002-5018-9126
Sem Percentil
26.00
Autor
QUITERO, MAYRA F.Z.
SIRIANI, LUCIANA K.
AZEVEDO, CYNTHIA S. de
FREITAS, ANDERSON Z. de
SCARAMUCCI, TAIS
SIMIONATO, MARIA R.L.
MATOS, ADRIANA B.
Resumen
This study was designed to investigate whether there is
a correlation between the findings of optical coherence
tomography (OCT) and polarized light microscopy (PLM)
when these techniques are used to evaluate standard
enamel white-spot lesions developed by distinct cariogenic
challenges. Bovine enamel fragments (N = 168)
were randomly allocated into 6 experimental groups
according to the microbiologic model (Streptococcus
mutans UA159, Streptococcus sobrinus ATCC 33478,
or mixed S mutans and S sobrinus) and carbohydrate
sources (1% sucrose or combined 1% sucrose and 1%
starch). Specimens were examined by OCT and PLM
every day within a period of 7 days. Five measurements
of demineralization depth were recorded for each specimen,
and means were calculated. Data were analyzed
with analysis of variance and Tukey tests (?? = 0.05), and
a correlation test was performed. All cariogenic challenges
created sub-superficial lesions. In both the OCT
and PLM analyses, the demineralization depth reached
its peak between days 6 and 7 of the cariogenic challenge,
except for the group challenged with S sobrinus
supplemented with combined sucrose and starch; for
that group, demineralization peaked on day 5 in the OCT
analysis. There was a significant correlation between
OCT and PLM (P = 0.00; r = 0.842). This preliminary study
suggests that OCT is a reliable, nondestructive method
to measure the demineralization depth of enamel whitespot
lesions, which can be useful for the laboratory and
has potential for clinical studies. Using the 1% sucrose
and S mutans model for 6 days is a simple and effective
method to induce enamel caries???like lesions without
compromising the depth and morphologic features of
the obtained lesions. Funda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP) FAPESP: 12/15141-6; 12/15089-4