Defining a suitable soil tillage option that provides adequate soil physical conditions for optimum cassava (Manihot esculenta Crantz) productivity has not been adequately researched in southern Brazil. This study aimed to evaluate, in an Argissolo Vermelho-Amarelo Distrófico (Acrisol or Hapludalf), three tillage methods - conventional (inverting) tillage, chiseling, and long-term no-tillage (without and with, additional soil compaction), as affecting soil hydro-physical properties and cassava yield, in southern Brazil. Undisturbed and disturbed soil samples were collected from row and interrow positions, from the soil surface down to 0.40 m depth to determine soil bulk density, degree of compaction, porosity, water retention, plant available water, air and water permeability, mechanical properties (compressibility and elasticity), and chemical properties. The yield of cassava storage roots was obtained at crop physiological maturity. Conventional (inverting) and chisel tillage of soil previously under long-term no-tillage increased soil macroporosity - a composition or capacity physical property – of the surface soil, but did not improve the functioning/intensity properties air and water permeability. Soil reconsolidation over a short-time significantly affects soil structural condition, and thus soil tillage is not needed to improve soil structure. Additional compaction on the no-till soil causes detrimental consequences on composition/capacity and functioning/ intensity physical properties. Nonetheless, neither improvement of soil structure by tillage nor further compaction affects cassava storage root yield in the sandy loam soil. Therefore, no-tillage is the best management system, in which soil loosening is done only during furrowing for cassava-stem planting.