Anastrepha Schiner is the genus of Tephritidae with the greatest diversity in the Neotropical region and the largest number of species in Brazil, and which imposes quarantine restrictions for imports of fruits and vegetables from countries in which it occurs. The objective of this paper is to present new records of Anastrepha species in State of Espírito Santo, Brazil. McPhail traps were used to capture adults in 74 municipalities. The occurrence of A. daciformis Bezzi, A. dissimilis Stone, A. mixta Zucchi and A. parallela (Wied.) are new records for the State of Espírito Santo.