Artigo
Treatment of soy milk with Debaryomyces hansenii cells immobilised in alginate
Autor
Souza Júnior, Waldeck Campanha de
Rezende, Sebastião Tavares de
Viana, Pollyanna Amaral
Falkoski, Daniel Luciano
Reis, Angélica Pataro
Machado, Solimar Gonçalves
Barros, Everaldo Gonçalves de
Guimarães, Valéria Monteze
Institución
Resumen
Whole cells of Debaryomyces hansenii UFV-1 were permeabilised with ethanol and immobilised in calcium alginate hydrogel. The optimum pH and temperature for α-galactosidase activities of permeabilised free (PFC) and permeabilised immobilised cells (PIC) were 4.5 and 60 °C; and 4.0 and 70 °C, respectively. PIC α-galactosidase was more stable than that of PFC. The incubation of PIC at 60 and 70 °C promoted an increase in α-galactosidase activity. The α-galactosidase activity was maintained when PIC was used in three repeated batches. The Km values for PIC and PFC α-galactosidases, with ρNPαGal, were 0.82 mM and 0.30 mM, respectively. Soy milk treatment with PIC for 6 h at 60 °C promoted 100% hydrolysis of raffinose oligosaccharides.