The potential role of companion animals as reservoirs for zoonotic diseases has been recognised as a significant public health problem worldwide. Ancylostoma ceylanicum is the only ancylostomatidae species known for infecting human beings. This article aimed to compare the predatory capacity of predatory fungi isolates Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34), Monacrosporium sinense (SF53) and Arthrobotrys robusta (I31) on A. ceylanicum infectious larvae (L3) in a 2% water–agar plate. There was no predatory capacity variation among the fungi tested (P > 0.05) over the 7-day period experimental assay. When compared to the control (without fungi), there was a significant reduction (P < 0.05) of 95.6%, 85.1%, 87.4% and 90.2% on the A. ceylanicum L3 mean recovered from treatments with isolates AC001, NF34, SF53 and I31, respectively. Regarding linear regression coefficients, negative values were noted for treatments, therefore indicating A. ceylanicum non-predated larvae reduction over 7 days. In this work, all predatory fungi isolates were efficient at capturing and destroying in vitro the A. ceylanicum L3; therefore being able to be used as biological controllers of such nematode.