Recovery of mutans streptococci on MSB, SB-20 and SB-20M agar media

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSaravia, Marta Estela
dc.creatorNelson-Filho, Paulo
dc.creatorSilva, Raquel Assed Bezerra
dc.creatorDe Rossi, Andiara
dc.creatorFaria, Gisele
dc.creatorSilva, Léa Assed Bezerra
dc.creatorEmilson, Claes-Göran
dc.date2014-05-27T11:28:33Z
dc.date2016-10-25T18:44:53Z
dc.date2014-05-27T11:28:33Z
dc.date2016-10-25T18:44:53Z
dc.date2013-03-01
dc.date.accessioned2017-04-06T02:14:53Z
dc.date.available2017-04-06T02:14:53Z
dc.identifierArchives of Oral Biology, v. 58, n. 3, p. 311-316, 2013.
dc.identifier0003-9969
dc.identifierhttp://hdl.handle.net/11449/74651
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74651
dc.identifier10.1016/j.archoralbio.2012.10.010
dc.identifierWOS:000315835200012
dc.identifier2-s2.0-84873738145
dc.identifierhttp://dx.doi.org/10.1016/j.archoralbio.2012.10.010
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895412
dc.descriptionObjective: The recovery of mutans streptococci in saliva and dental biofilm samples depends, in part, on the culture medium used. In this study, we compared (i) the culture media Sucrose-Bacitracin agar (SB-20), Modified SB-20 (SB-20M) and Mitis Salivarius Bacitracin agar (MSB) in the count of colony forming units (cfu) of mutans streptococci and (ii) in the morphological and biochemical differentiation between Streptococcus mutans and Streptococcus sobrinus. Design: Samples of non-stimulated saliva from 20 children were plated on SB-20, SB-20M and MSB, and incubated in microaerophilia at 37 °C for 72 h. Identification of microorganisms was based on analysis of colony morphology under stereomicroscopy. The biochemical identification of colonies was done by biochemical tests using sugar fermentation, resistance to bacitracin and hydrogen peroxide production. Results: There was no significant difference (p > 0.05) in the number of cfu of mutans streptococci recovered on SB-20 and SB-20M agar. Comparing the media, SB-20 and SB-20M yielded a larger number of mutans streptococci colonies (p < 0.05) and were more effective than MSB in the identification of S. sobrinus (p < 0.05), but not of S. mutans (p > 0.05). Conclusion: There was no significant difference between SB-20 and SB-20M culture media in the count of mutans streptococci, demonstrating that the replacement of sucrose by coarse granular cane sugar did not alter the efficacy of the medium. Compared with MSB, SB-20 and SB-20M allowed counting a larger number of mutans streptococci colonies and a more effective morphological identification of S. sobrinus. © 2012 Elsevier Ltd.
dc.languageeng
dc.relationArchives of Oral Biology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSelective culture media
dc.subjectStreptococcus mutans
dc.subjectStreptococcus sobrinus
dc.subjectagar
dc.subjectantiinfective agent
dc.subjectbacitracin
dc.subjectsucrose
dc.subjectantibiotic resistance
dc.subjectbacterial load
dc.subjectbacterium identification
dc.subjectbiofilm
dc.subjectchild
dc.subjectclassification
dc.subjectcomparative study
dc.subjectculture medium
dc.subjectfermentation
dc.subjecthuman
dc.subjectisolation and purification
dc.subjectmetabolism
dc.subjectmicrobiological examination
dc.subjectmicrobiology
dc.subjectpreschool child
dc.subjectsaliva
dc.subjectAgar
dc.subjectAnti-Bacterial Agents
dc.subjectBacitracin
dc.subjectBacterial Load
dc.subjectBacterial Typing Techniques
dc.subjectBacteriological Techniques
dc.subjectBiofilms
dc.subjectChild
dc.subjectChild, Preschool
dc.subjectCulture Media
dc.subjectDrug Resistance, Bacterial
dc.subjectFermentation
dc.subjectHumans
dc.subjectSaliva
dc.subjectSucrose
dc.subjectStreptococcus
dc.titleRecovery of mutans streptococci on MSB, SB-20 and SB-20M agar media
dc.typeOtro


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