Brasil | Otro

Primordial germ cells (spermatogonial stem cells) of bullfrogs express sex hormone-binding globulin and steroid receptors during seasonal spermatogenesis

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCaneguim, Breno Henrique
dc.creatorBeltrame, Flávia Luciana
dc.creatorDa Luz, Juliana Silva
dc.creatorValentini, Sandro Roberto
dc.creatorCerri, Paulo Sérgio
dc.creatorCerri, Estela Sasso
dc.date2014-05-27T11:27:30Z
dc.date2016-10-25T18:41:06Z
dc.date2014-05-27T11:27:30Z
dc.date2016-10-25T18:41:06Z
dc.date2013-01-01
dc.date.accessioned2017-04-06T02:07:48Z
dc.date.available2017-04-06T02:07:48Z
dc.identifierCells Tissues Organs, v. 197, n. 2, p. 136-144, 2013.
dc.identifier1422-6405
dc.identifier1422-6421
dc.identifierhttp://hdl.handle.net/11449/74237
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74237
dc.identifier10.1159/000341517
dc.identifierWOS:000314013800005
dc.identifier2-s2.0-84872674987
dc.identifierhttp://dx.doi.org/10.1159/000341517
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895009
dc.descriptionIn vertebrate species, testosterone seems to inhibit spermatogonial differentiation and proliferation. However, this androgen can also be converted, via aromatase, into estrogen which stimulates spermatogonial differentiation and mitotic activity. During seasonal spermatogenesis of adult bullfrogs Lithobates catesbeianus, primordial germ cells (PGCs) show enhanced testosterone cytoplasm immunoexpression in winter; however, in summer, weak or no testosterone immunolabelling was observed. The aim of this study was to confirm if PGCs express stem cell markers-alkaline phosphatase (AP) activity and GFRα1 (glial-cell-line-derived neurotrophic factor)-and verify whether testosterone is maintained in these cells by androgen receptors (ARs) and/or sex hormone-binding globulin (SHBG) in winter. Furthermore, regarding the possibility that testosterone is converted into estrogen by PGCs in summer, the immunoexpression of estrogen receptor (ER)β was investigated. Bullfrog testes were collected in winter and in summer and were embedded in glycol methacrylate for morphological analyses or in paraffin for the histochemical detection of AP activity. GFRα1, AR, SHBG and ERβ expression were detected by Western blot and immunohistochemical analyses. The expression of AP activity and GFRα1 in the PGCs suggest that these cells are spermatogonial stem cells. In winter, the cytoplasmic immunoexpression of ARs and SHBG in the PGCs indicates that testosterone is maintained by these proteins in these cells. The cytoplasmic immunoexpression of ERβ, in summer, also points to an ER-mediated action of estrogen in PGCs. The results indicate a participation of testosterone and estrogen in the control of the primordial spermatogonia during the seasonal spermatogenesis of L. catesbeianus. © 2012 S. Karger AG, Basel.
dc.languageeng
dc.relationCells Tissues Organs
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAdult bullfrogs
dc.subjectAndrogen and estrogen receptors
dc.subjectPrimordial germ cells
dc.subjectSeasonal spermatogenesis
dc.subjectStem spermatogonia
dc.subject2 hydroxyethyl methacrylate
dc.subjectalkaline phosphatase
dc.subjectandrogen receptor
dc.subjectestrogen
dc.subjectestrogen receptor beta
dc.subjectglial cell line derived neurotrophic factor
dc.subjectparaffin
dc.subjectsex hormone binding globulin
dc.subjecttestosterone
dc.subjectanimal cell
dc.subjectbullfrog
dc.subjectcytoplasm
dc.subjectenzyme activity
dc.subjecthistochemistry
dc.subjectimmunohistochemistry
dc.subjectmale
dc.subjectnonhuman
dc.subjectprimordial germ cell
dc.subjectpriority journal
dc.subjectspermatogenesis
dc.subjectsummer
dc.subjecttestis
dc.subjectWestern blotting
dc.subjectwinter
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectEstrogen Receptor beta
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectMale
dc.subjectRana catesbeiana
dc.subjectReceptors, Androgen
dc.subjectSex Hormone-Binding Globulin
dc.subjectSpermatogenesis
dc.subjectSpermatogonia
dc.subjectStem Cells
dc.subjectLithobates
dc.subjectVertebrata
dc.titlePrimordial germ cells (spermatogonial stem cells) of bullfrogs express sex hormone-binding globulin and steroid receptors during seasonal spermatogenesis
dc.typeOtro


Este ítem pertenece a la siguiente institución