Accidental infection by Trypanosoma cruzi (T. cruzi) usually occurs through accident with a sharp instrument during handling of infected animals in research laboratories. This case report describes the evolution of a case of accidentally acquired acute Chagas disease (CD). A 46-year-old female with suspicious symptoms of acute CD received medical care after reported a sharp puncture accident, during a laboratory procedure with an animal contaminated with T. cruzi. The direct search for T. cruzi in blood smear was positive. Benznidazole (BZN) 400 mg daily was started. The patient evolved with prompt remission of symptoms and completed 70 consecutive days of BZN with no adverse reactions, achieving parasitological, serological, and clinical cure within 6 months.