Extracellular vesicle microRNAs in celiac disease patients under a gluten-free diet, and in lactose intolerant individuals

dc.creatorLemos, Débora S.
dc.creatorBeckert, Helen C.
dc.creatorOliveira, Luana C.
dc.creatorBerti, Fernanda C. B.
dc.creatorOzawa, Patricia M.M.
dc.creatorSouza, Ingrid L.M.
dc.creatorZanata, Silvio M.
dc.creatorPankievicz, Vânia C.S.
dc.creatorTuleski, Thalita R.
dc.creatorSouza, Emanuel M.
dc.creatorSilva, Rosiane V.
dc.creatorWowk, Pryscilla F.
dc.creatorPetzl-Erler, Maria Luiza
dc.creatorAlmeida, Rodrigo C.
dc.creatorCipolla, Gabriel Adelman
dc.creatorBoldt, Angelica B.W.
dc.creatorMalheiros, Danielle
dc.date2023-05-17T12:49:45Z
dc.date2023-05-17T12:49:45Z
dc.date2022
dc.date.accessioned2023-09-27T00:13:35Z
dc.date.available2023-09-27T00:13:35Z
dc.identifierLEMOS, Débora S. et al. Extracellular vesicle microRNAs in celiac disease patients under a gluten-free diet, and in lactose intolerant individuals. BBA Advanced, v. 2, p. 1-10, 2022.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/58455
dc.identifierhttps://doi.org/10.1016/j.bbadva.2022.100053
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898706
dc.descriptionCeliac disease (CD) is an autoimmune disorder triggered by an abnormal immunological response to gluten ingestion and is associated with deregulated expression of cellular microRNAs (miRNAs) of the gut mucosa. It is frequently misdiagnosed as lactose intolerance (LI) due to symptom resemblance. Microvilli loss may be counteracted by a rigorous gluten-free diet (GFD). Aims: To identify altered extracellular vesicle miRNAs from plasma among CD patients on GFD (n=34), lactose intolerant individuals on restrictive diet (n=14) and controls (n=23), and to predict biological pathways in which these altered miRNAs may play a part. Methods: Five different small RNA samples of each group were pooled twice and then screened by new-generation sequencing. Four miRNAs were selected to be quantified by RT-qPCR in the entire sample. Results: The levels of four miRNAs – miR-99b-3p, miR-197-3p, miR-223-3p, and miR-374b-5p – differed between CD patients and controls (P<0.05). Apart from miR-223-3p, all these miRNAs tended to have altered levels also between LI and controls (P<0.10). The results for miR-99b-3p and miR-197-3p between CD and controls were confirmed by RT-qPCR, which also indicated different levels of miR-99b-3p and miR-374b-5p between CD-associated LI and LI(P<0.05). Conclusions: These miRNAs may have targets that affect cell death, cell communication, adhesion, and inflammation modulation pathways. Hence, altered miRNA levels could be associated with CD-related aspects and gut mucosa recovery.
dc.formatapplication/pdf
dc.languagepor
dc.publisherElsevier
dc.rightsopen access
dc.subjectCeliac Disease
dc.subjectLactose Intolerance
dc.subjectExtracellular Vesicles
dc.subjectMicroRNAs
dc.subjectDiet, Gluten-Free
dc.subjectEnfermedad Celíaca
dc.subjectIntolerancia a la Lactosa
dc.subjectVesículas Extracelulares
dc.subjectMicroARNs
dc.subjectDieta Sin Gluten
dc.subjectMaladie coeliaque
dc.subjectIntolérance au lactose
dc.subjectVésicules extracellulaires
dc.subjectmicroARN
dc.subjectRégime sans gluten
dc.subjectDoença Celíaca
dc.subjectIntolerância à Lactose
dc.subjectVesículas Extracelulares
dc.subjectMicroRNAs
dc.subjectDieta Livre de Glúten
dc.titleExtracellular vesicle microRNAs in celiac disease patients under a gluten-free diet, and in lactose intolerant individuals
dc.typeArticle


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