Immune Response to the Rhodococcus equi infection in high and low antibody-producing mice (selection IV-A)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPedrini, Sílvia C. B.
dc.creatorAcorci, Michele J.
dc.creatorPinto, João G. G.
dc.creatorSilveira, Liciana V. A.
dc.creatorOliveira, Silvio L.
dc.date2014-05-27T11:21:40Z
dc.date2016-10-25T18:21:18Z
dc.date2014-05-27T11:21:40Z
dc.date2016-10-25T18:21:18Z
dc.date2005-11-10
dc.date.accessioned2017-04-06T01:15:21Z
dc.date.available2017-04-06T01:15:21Z
dc.identifierMicrobiology and Immunology, v. 49, n. 10, p. 915-923, 2005.
dc.identifier0385-5600
dc.identifier1348-0421
dc.identifierhttp://hdl.handle.net/11449/68490
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68490
dc.identifier10.1111/j.1348-0421.2005.tb03683.x
dc.identifierWOS:000232434600005
dc.identifier2-s2.0-27544474216
dc.identifierhttp://dx.doi.org/10.1111/j.1348-0421.2005.tb03683.x
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889822
dc.descriptionRhodococcus equi is a Gram-positive, facultative intracellular bacterium which infects macrophages and causes rhodococcal pneumonia and enteritis in foals. Recently, this agent has been recognized as an opportunistic pathogen for immunocompromised humans. Several murine experimental models have been used to study R. equi infection. High (H IV-A) and Low (L IV-A) antibody (Ab)-producers mice were obtained by bi-directional genetic selections for their ability to produce antibodies against sheep and human erythrocytes (Selection IV-A). These lines maintain their phenotypes of high and low responders also for other antigens than those of selection (multispeciflc effect). A higher macrophage activity in L IV-A mice has been described for several intracellular infectious agents, which could be responsible for their intense macrophage antigens (Ag)-handling and low Ab production. Due to these differences, L IV-A mice were found to exhibit a better performance to trigger an effective immune response towards intracellular pathogens. The objective of this work was to characterize the immune response of Selection IV-A against R. equi. H IV-A and L IV-A mice were infected with 2.0 × 10 6 CFU of ATCC 33701 +R. equi by intravenous route. With regards to bacterial clearance and survival assays, L IV-A mice were more resistant than H IV-A mice to virulent R. equi. L IV-A mice presented a higher hydrogen peroxide (H 2O 2) and nitric oxide (NO) endogenous production by splenic macrophages than H IV-A mice. L IV-A expressed the most intense cellular response, available by the Delayed-Type Hypersensitivity (DTH) reaction, which activated macrophages and produced more H 2O 2 and NO. The three times higher specific antibodies titres in H IV-A indicated that Selection IV-A maintained the multispecific effect and the polygenic control of humoral and cellular responses also to R. equi.
dc.languageeng
dc.relationMicrobiology and Immunology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiozzi mice
dc.subjectImmune response
dc.subjectMacrophage
dc.subjectRhodococcus equi
dc.subjectantibody
dc.subjectantigen
dc.subjecthydrogen peroxide
dc.subjectnitric oxide
dc.subjectanimal model
dc.subjectantibody production
dc.subjectbacterial infection
dc.subjectcell activity
dc.subjectcellular immunity
dc.subjectcontrolled study
dc.subjectenteritis
dc.subjecterythrocyte
dc.subjectfemale
dc.subjectgenetics
dc.subjecthumoral immunity
dc.subjecthypersensitivity reaction
dc.subjectimmune deficiency
dc.subjectimmune response
dc.subjectmacrophage
dc.subjectmouse
dc.subjectnonhuman
dc.subjectphenotype
dc.subjectpneumonia
dc.subjectsheep
dc.subjectspleen cell
dc.subjectsurvival
dc.subjecttitrimetry
dc.subjectvirulence
dc.subjectActinomycetales Infections
dc.subjectAnimals
dc.subjectAntibodies, Bacterial
dc.subjectColony Count, Microbial
dc.subjectDisease Models, Animal
dc.subjectFemale
dc.subjectHydrogen Peroxide
dc.subjectHypersensitivity, Delayed
dc.subjectLiver
dc.subjectLung
dc.subjectMacrophages
dc.subjectMice
dc.subjectNitric Oxide
dc.subjectSpleen
dc.subjectSurvival Analysis
dc.subjectAnimalia
dc.subjectBacteria (microorganisms)
dc.subjectMurinae
dc.subjectOvis aries
dc.subjectPosibacteria
dc.titleImmune Response to the Rhodococcus equi infection in high and low antibody-producing mice (selection IV-A)
dc.typeOtro


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