Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil

dc.creatorChalhoub, Flávia Löwen Levy
dc.creatorHorta, Marco Aurélio Pereira
dc.creatorAlcantara, Luiz Carlos Junior
dc.creatorMorales, Alejandra
dc.creatorSantos, Lilha Maria Barbosa dos
dc.creatorCampos, Vinícius Guerra
dc.creatorRodrigues, Cintia D.S.
dc.creatorSantos, Carolina C.
dc.creatorMares-Guia, Maria Angélica M.
dc.creatorPauvolid-Corrêa, Alex
dc.creatorFilippis, Ana Maria Bispo de
dc.date2023-01-17T11:19:22Z
dc.date2023-01-17T11:19:22Z
dc.date2022
dc.date.accessioned2023-09-27T00:08:20Z
dc.date.available2023-09-27T00:08:20Z
dc.identifierCHALHOUB, Flávia Löwen Levy et al. Serological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil. Viruses. v. 14, 2459, p. 1 - 19, Nov. 2022.
dc.identifier1999-4915
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/56481
dc.identifier10.3390/v14112459
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8897816
dc.descriptionInfections with arboviruses are reported worldwide. Saint Louis encephalitis (SLEV) and West Nile (WNV) viruses are closely related flaviviruses affecting humans and animals. SLEV has been sporadically detected in humans, and corresponding antibodies have been frequently detected in horses throughout Brazil. WNV was first reported in western Brazil over a decade ago, has been associated with neurological disorders in humans and equines and its prevalence is increasing nationwide. Herein, we investigated by molecular and serological methods the presence of SLEV and WNV in equines from Rio de Janeiro. A total of 435 serum samples were collected from healthy horses and tested for specific neutralizing antibodies by plaque reduction neutralization test (PRNT90). Additionally, samples (serum, cerebrospinal fluid, central nervous system tissue) from 72 horses, including horses with neurological disorders resulting in a fatal outcome or horses which had contact with them, were tested by real-time reverse transcription–polymerase chain reaction (RT-qPCR) for both viruses. Adopting the criterion of four-fold antibody titer difference, 165 horses (38%) presented neutralizing antibodies for flaviviruses, 89 (20.4%) for SLEV and five (1.1%) for WNV. No evidence of SLEV and WNV infection was detected by RT-qPCR and, thus, such infection could not be confirmed in the additional samples. Our findings indicate horses of Rio de Janeiro were exposed to SLEV and WNV, contributing to the current knowledge on the distribution of these viruses in Brazil.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectVírus do Nilo Ocidental
dc.subjectVírus da encefalite de Saint Louis
dc.subjectCavalos
dc.subjectTeste de neutralização de redução de placa
dc.subjectRT-qPCR
dc.subjectRio de Janeiro
dc.subjectBrasil
dc.subjectWest Nile virus
dc.subjectSaint Louis encephalitis virus
dc.subjectHorses
dc.subjectPlaque reduction neutralization test
dc.subjectRT-qPCR
dc.subjectRio de Janeiro
dc.subjectBrazil
dc.titleSerological Evidence of Exposure to Saint Louis Encephalitis and West Nile Viruses in Horses of Rio de Janeiro, Brazil
dc.typeArticle


Este ítem pertenece a la siguiente institución