Article
Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG+874 A>T polymorphism
Registro en:
CARNEIRO, V. L. et al. Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG+874 A>T polymorphism. Brazilian Journal of Infectious Diseases, v. 22, n. 3, p. 202–207, 2018.
1413-8670
10.1016/j.bjid.2018.04.004
Autor
Carneiro, Valdirene Leão
Bendicho, Maria Teresita
Santos, Rosalina Guedes
Casela, Marilda
Martins Netto, Eduardo
Mota, Scarlet Torres Moraes
Pina, Iza Cristina Araújo
Nascimento, Roberto Meyer
Freire, Songeli Menezes
Barbosa, Theolis Costa
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (INCT-DT/MCT/CNPq 573839/2008-5) and Fundação de Amparo à Pesquisa do Estado da Bahia (PET035/2013). Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG+874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG+874 A/T polymorphism on interferon-gamma test performance. Methods: Patients diagnosed with pulmonary tuberculosis (75), volunteers with positivetuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no his-tory of contact with tuberculosis (57) were evaluated regarding the IFNG + 874 genotype andthe IFN- levels in whole blood cultures performed using an interferon-gamma commercialkit (QuantiFERON-TB®Gold In-Tube).Results: IFN- production was not influenced by the IFNG + 874 genotype, regardless of anti-gen or mitogen-based stimulation, which suggests that other genes may influence IFN- production in response to mycobacteria. The IFNG + 874 polymorphism was found to exertno influence over QFT-IT test sensitivity in our study.