The ceja-1 sequence as a potential new molecular marker for Paracoccidioides brasiliensis infection

dc.creatorCorreia, Janaina
dc.creatorBorba, Cíntia de Moraes
dc.creatorReis, Beatriz
dc.creatorMartins, Albert
dc.creatorUnkles, Sheila
dc.creatorKinghorn, James Robert
dc.creatorLucena-Silva, Norma
dc.date2018-05-07T12:43:14Z
dc.date2018-05-07T12:43:14Z
dc.date2010
dc.date.accessioned2023-09-26T23:58:56Z
dc.date.available2023-09-26T23:58:56Z
dc.identifierCORREIA, J. et al. The ceja-1 sequence as a potential new molecular marker for Paracoccidioides brasiliensis infection. Mycoses, v. 53, n. 2, p. 130–137, 1 mar. 2010.
dc.identifier1439-0507
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/26299
dc.identifier10.1111/j.1439-0507.2008.01682.x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8896215
dc.descriptionWe have developed a two-step PCR assay that amplifies a region of the ceja-1 sequence that is specific for virulent strains of Paracoccidioides brasiliensis. An internal region of the ceja-1 sequence was chosen for designing primers that were utilised in a single tube heminested PCR protocol to amplify DNA from six virulent strains. PCR specificity was determined by the absence of amplified products with genomic DNA from four non-virulent strains of P. brasiliensis and from eight fungal pathogens, one bacterium, two protozoa, one worm and mouse and human genomic DNA (leucocytes). The fact that the PCR product was only obtained with the genetic material from virulent isolates of P. brasiliensis suggested that this partial amplified sequence might be a marker of virulence for this fungus. The diagnostic potential of this PCR was confirmed by the successful amplification of this fragment with genomic DNA obtained in lymph node aspirate from a patient with paracoccidioidomycosis.
dc.description2050-01-01
dc.formatapplication/pdf
dc.languageeng
dc.rightsrestricted access
dc.subjectParacoccidioides brasiliensis
dc.subjectVirulência
dc.subjectPCR hemineste
dc.subjectParacoccidioides brasiliensis
dc.subjectVirulence
dc.subjectHeminested PCR
dc.subjectAnimais
dc.subjectBiomarcadores
dc.subjectPrimers DNA / genética
dc.subjectDNA, Fúngico / química
dc.subjectDNA Fúngico / genética
dc.subjectHumanos
dc.subjectRatos
dc.subjectDados de Sequencia Molecular
dc.subjectMicologia / métodos
dc.subjectParacoccidioides / genética
dc.subjectParacoccidioides / isolamento e purificação
dc.subjectParacoccidioidomicose / diagnóstico
dc.subjectReação em Cadeia da Polimerase / métodos
dc.subjectSensibilidade e Especificidade
dc.subjectAnálise de Sequência, DNA
dc.titleThe ceja-1 sequence as a potential new molecular marker for Paracoccidioides brasiliensis infection
dc.typeArticle


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