Article
Anopheles darlingi polytene chromosomes: revised maps including newly described inversions and evidence for population structure in Manaus
Registro en:
CORNEL, Anthony J. et al. Anopheles darlingi polytene chromosomes: revised maps including newly described inversions and evidence for population structure in Manaus. Memórias do Instituto Oswaldo Cruz, v. 111, n. 5, p. 335-346, May 2016.
0074-0276
10.1590/0074-02760150470
1678-8060
Autor
Cornel, Anthony J.
Brisco, Katherine K.
Tadei, Wanderli Pedro
Secundino, Nágila Francinete Costa
Rafael, Miriam Silva
Galardo, Allan Kardec Ribeiro
Medeiros, Jansen Fernandes de
Pessoa, Felipe Arley Costa
Ríos-Velásquez, Claudia María
Lee, Yoosook
Pimenta, Paulo Filemon Paolucci
Lanzaro, Gregory C.
Resumen
CAPES CNPq FAPEAM FAPEMIG FIOCRUZ PAPES Science Without Borders Program Salivary gland polytene chromosomes of 4th instar Anopheles darlingi Root were examined from multiple locations in the Brazilian Amazon. Minor modifications were made to existing polytene photomaps. These included changes to the breakpoint positions of several previously described paracentric inversions and descriptions of four new paracentric inversions, two on the right arm of chromosome 3 and two on the left arm of chromosome 3 that were found in multiple locations. A total of 18 inversions on the X (n = 1) chromosome, chromosome 2 (n = 7) and 3 (n = 11) were scored for 83 individuals from Manaus, Macapá and Porto Velho municipalities. The frequency of 2Ra inversion karyotypes in Manaus shows significant deficiency of heterozygotes (p < 0.0009). No significant linkage disequilibrium was found between inversions on chromosome 2 and 3. We hypothesize that at least two sympatric subpopulations exist within the An. darlingi population at Manaus based on inversion frequencies.