Article
Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma
Registro en:
DANTAS, Rebeca C.M. et al. Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma. Applied Immunohistochemistry and Molecular Morphology, 2020.
1541-2016
Autor
Dantas, Rebeca C. M.
Guimarães, Vanessa Sousa Nazaré
Souza, Renata O. de
Valverde, Ludmila de Faro
Vidal, Manuela T. A.
Nogueira, Raphael L. R.
Rocha, Leonardo O. S. da
Araújo, Giovana T.
Santos, Jean N. dos
Rocha, Clarissa Araújo Gurgel
Resumen
In oral squamous cell carcinoma (OSCC), involvement
and activation of the Hedgehog pathway (HH) may be related to
epithelial-mesenchymal transition and cell proliferation. The present
study aimed to evaluate epithelial-mesenchymal transition and
proliferative potential in OSCC cases demonstrating activation of
the HH pathway. Twenty-three GLi-1-positive OSCC cases were
submitted to immunohistochemical detection of Snail, Slug,
N-cadherin, E-cadherin, β-catenin, and MCM3 proteins. Clinicalpathologic
immunoexpression data were obtained from the invasion
front and tumor islets, and then compared. At the invasion
front, OSCC cases presented positive Snail, Slug, and MCM3 expression
in the nuclei of tumor cells. Loss of membrane and cytoplasmic
expression of E-cadherin and β-catenin was also observed.
Positive N-cadherin expression was observed in 31.78% of the cases.
GLi-1 immunoexpression was associated with loss of membrane
E-cadherin (P<0.001), membrane β-catenin (P<0.001), and cytoplasmic
β-catenin (P=0.02) expression. In the tumor islets,
we observed nuclear expression of GLi-1, Snail, Slug, and
MCM3. E-cadherin and β-catenin showed positivity in tumor cell
membranes. Statistically significant positive correlations between
GLi-1 and Snail (P=0.05), E-cadherin (P=0.01), and cytoplasmic
β-catenin (P=0.04) were found. GLi-1 was associated with clinical
staging, while membrane β-catenin expression was related to
the presence of metastasis in lymph nodes and to clinical staging.
The HH pathway may be involved in regulating the expression of
the mesenchymal phenotype. The loss of membrane E-cadherin and
β-catenin expression was observed at the tumor front region,
whereas cell adhesion protein expression was detected in tumor
islets regardless of MCM3.