Article
Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain
Registro en:
ROSE, Tatiana L.; MIAGOSTOVICH, Marize P.; LEITE, José Paulo G. Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain. Mem Inst Oswaldo Cruz, v.105, n.8, p.1068-1072, Dec. 2010.
0074-0276
1678-8060
Autor
Rose, Tatiana L.
Miagostovich, Marize P.
Leite, José Paulo G.
Resumen
Rotaviruses are important enteric pathogens for humans and animals. Group A rotaviruses (RV-A) are the
most common agents of severe gastroenteritis in infants and young children and vaccination is the most effective
method to reduce RV-A-associated diseases. G1P[8], the most prevalent RV-A genotype worldwide, is included
in the RV-A vaccine Rotarix®. The discrimination between wild-type G1P[8] and vaccine G1P[8] strains is an
important topic in the study of RV-A epidemiology to manage outbreaks and to define control measures for vaccinated
children. In this study, we developed a novel method to segregate the wild-type and vaccine strains using
restriction endonucleases. The dsRNA from the Rotarix® vaccine was sequenced and the NSP3 gene was selected
as the target gene. The vaccine strain has a restriction pattern that is different than that of wild-type RV-A G1P[8]
isolates after digestion with the restriction endonuclease BspHI. This pattern could be used as a marker for the
differentiation of wild-type G1P[8] strains from the vaccine strain.
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