Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.

dc.creatorMiranda, G. Cabral
dc.creatorJesus, Joílson Ramos de
dc.creatorOliveira, Pablo Rafael Silveira
dc.creatorBritto, G. S. G
dc.creatorPontes-de-Carvalho, Lain Carlos
dc.creatorDutra, R. F
dc.creatorNeves, Neuza Maria Alcântara
dc.date2014-06-04T17:37:34Z
dc.date2014-06-04T17:37:34Z
dc.date2014
dc.date.accessioned2023-09-26T23:47:21Z
dc.date.available2023-09-26T23:47:21Z
dc.identifierCABRAL-MIRANDA, G. et al.Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors. Journal of Parasitology, v. 100, n. 1, p. 73-78, 2014.
dc.identifier1937-2345
dc.identifier10.1645/GE-3052.1
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/7791
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8894224
dc.descriptionDiseases such as leishmaniases are important causes of morbidity and mortality in Brazil, and their diagnoses need to be improved. The use of monoclonal antibodies has ensured high specificity to immunodiagnosis. The development of an immunosensor, coupling a monoclonal antibody to a bioelectronic device capable of quickly detecting Leishmania sp. antigens both qualitatively and quantitatively, is a promising alternative for the diagnosis of leishmaniasis due to its high specificity, low cost, and portability, compared with conventional methods. The present work was aimed at developing an immunosensor-based assay for detecting Leishmania infantum antigens in tissues of infected hosts. Four hybridomas producing monoclonal antibodies against L. infantum had their specificity confirmed by enzyme-linked immunosorbent assay. These antibodies were immobilized on a gold surface, covered with a thin film of 2-aminoethanethiol (cysteamine) and glutaraldehyde, blocked with glycine, and placed into contact with extracts of L. infantum–infected and noninfected control hamster spleens. The assay was able to detect 1.8 3 104 amastigotes/g of infected tissue. These results demonstrated that this assay may be useful for quantifying L. infantum amastigotes in organs of experimental animals for studies on pathogenesis and immunity and that it is a promising tool for the development of a diagnostic method, based on antigen detection, of human and dog visceral leishmaniasis.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society of Parasitologists
dc.rightsopen access
dc.subjectAnticorpos Monoclonais/uso diagnóstico
dc.subjectAntígenos de Protozoários/isolamento & purificação
dc.subjectLeishmania infantum/imunologia
dc.subjectLeishmaniose Visceral/diagnóstico
dc.subjectBaço/parasitologia
dc.subjectAnimais
dc.subjectAnticorpos Monoclonais/imunologia
dc.subjectAnticorpos Antiprotozoários/uso diagnóstico
dc.subjectAnticorpos Antiprotozoários/imunologia
dc.subjectEspecificidade de Anticorpos
dc.subjectTécnicas Biossensoriais/métodos
dc.subjectCricetinae
dc.subjectEnsaio de Imunoadsorção Enzimática
dc.subjectImunoglobulina G/uso diagnóstico
dc.subjectLeishmania infantum/isolamento & purificação
dc.subjectMesocricetus
dc.subjectCamundongos
dc.subjectCamundongos Endogâmicos BALB C
dc.subjectOrganismos Livres de Patógenos Específicos
dc.subjectBaço/imunologia
dc.titleDetection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.
dc.typeArticle


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