Article
Molecular detection of Mansonella mariae incriminates Simulium oyapockense as a potentially important bridge vector for Amazon-region zoonoses
Registro en:
SILVA, Túllio Romão Ribeiro da et al. Molecular detection of Mansonella mariae incriminates Simulium oyapockense as a potentially important bridge vector for Amazon-region zoonoses. Infection, Genetics and Evolution, v. 98, 105200, p. 1 - 3, Jan. 2022.
1567-1348
10.1016/j.meegid.2021.105200
Autor
Silva, Túllio Romão Ribeiro da
Narzetti, Luiz Henrique de Aquino
Crainey, James Lee
Costa, Carlos Henrique
Santos, Yago Vinícius Serra dos
Leles, Lorena Ferreira de Oliveira
Pessoa, Felipe Arley Costa
Vicente, Ana Carolina Paulo
Luz, Sérgio Luiz Bessa
Resumen
Objective: To assess the emergent zoonotic disease risk posed by the voracious human-biting blackfly species
Simulium oyapockense in the peripheral regions of an expanding urban centre situated deep in the Brazilian
Amazon rainforest.
Methods: We performed nine human landing catches at three periurban sites surrounding the Brazilian Amazon
town of S˜ao Gabriel da Cachoeira. Using the detection of non-human primate filarial parasites as an indicator of
the zoonotic disease threat posed by a biting insect, we screened 3328 S. oyapockense blackflies for the presence
of zoonotic filarial DNA with an ITS-1 PCR assay and Sanger sequencing.
Results: Between 98 and 100% of the biting insects captured during our nine collections were identified as
S. oyapockense; at our three collection sites and during our three seasonally-distinct collections this species was
captured at rates between 28 and 294 blackflies per hour. PCR screening of the march-collected S. oyapockense
detected infectious-stage (L3) Mansonella mariae parasites (which are only known to infect non-human primates)
in >0.15% of the tested head samples.
Conclusions: Our results show that residents of the periurban regions of S˜ao Gabriel da Cachoeira are routinely
exposed to the bites of S. oyapockense blackflies which have previously fed on non-human primates.