Article
Identification of potential biomarkers for systemic lupus erythematosus diagnosis using twodimensional differential gel electrophoresis (2DDIGE) and mass spectrometry
Registro en:
FERREIRA, Tamara Aparecida Reis; et al. Identification of potential biomarkers for systemic lupus erythematosus diagnosis using two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry. Autoiimunity, v.50, n.4, p.247-256, 2017.
0891-6934
10.1080/08916934.2017.1344975
1607-842X
Autor
Ferreira, Tamara Aparecida Reis
Andrade, Hélida Monteiro de
Pádua, Paulo Madureira de
Carvalho, Maria das Graças
Pires, Simone da Fonseca
Oliveira, Ivana Helena Rocha
Lima, Bruna Soares Souza
Fialho Junior, Luis Carlos
Cicarini, Walter Batista
Chapeourouge, Donat Alexander
Perales, Jonas Henrique
Guimarães, Tânia Mara Pinto Dabés
Toledo, Vicente de Paulo Coelho Peixoto de
Resumen
Systemic lupus erythematosus (SLE) is an autoimmune disease of the connective tissue with a large
spectrum of clinical manifestations. Immune deregulation leads to autoantibody and immune complexes
overproduction, complement activation, and persistent tissue inflammation. Considering that
the current diagnosis depends on the interpretation of the complex criteria established by the
American College of Rheumatology and that the disease course is characterized by unpredictable activations
and remissions, each patient develops different manifestations, and therefore, the discovery of
specific biomarkers is urgently required. Therefore, this study aimed to identify putative biomarkers for
active and inactive SLE potentially capable in distinguishing laboratorial SLE from other autoimmune
diseases. The 2D-DIGE proteomics technique was used to evaluate the differential abundance of proteins
between patients with active SLE, inactive SLE, patients with other autoimmune disease, and
healthy individuals. Six proteins showed increased abundance in active SLE (A) and inactive SLE (I)
compared to the C and O groups, but not between groups A and I. There were two transthyretin (TTR)
fragments or proteins with a structure similar to TTR (accession numbers: PDB: 1GKO_A and 2PAB_A),
retinol-binding protein 4 (RBP4) isoform X1 (no information in databases such as UNIPROT), and antibody
fragments. Two proteins, APO-AIV and SP-40,40, were upregulated in group A than in O and C
and in group I versus C, but not in group I versus O. Therefore, we suggest these proteins to be considered
as candidates for the diagnosis of SLE. 2030-01-01