Article
Detection of virulence and antibiotic resistance genes in environmental strains of Vibrio spp. from mussels along the coast of Rio de Janeiro State, Brazil
Registro en:
OLIVA, Marcelo Santos de; et al. Detection of virulence and antibiotic resistance genes in environmental strains of Vibrio spp. from mussels along the coast of Rio de Janeiro State, Brazil. African Journal of Microbiology Research, v.10, n.24, p.906-913, June 2016.
1996-0808
10.5897/AJMR2015.7636
Autor
Oliva, Marcelo Santos de
Bronzato, Greiciane França
Soares, Lidiane de Castro
Pereira, Ingrid Annes
Pribul, Bruno Rocha
Souza, Marco Antônio Soares de
Coelho, Shana Mattos de Oliveira
Rodrigues, Dália dos Prazeres
Souza, Miliane Moreira Soares de
Resumen
Mussels have a filter system enabling them to take up nutrients from the water, so a microbiological
analysis of these bivalve mollusks can show the contamination levels of their surrounding aquatic
environment. The present work aimed to isolate Vibrio species from two hundred samples of mussels
(Perna perna) incrusted on rocks of the Santana Archipelago and from longline mariculture in Ilha
Grande Bay in Angra dos Reis and from Arraial do Cabo, all of which are in Rio de Janeiro state, Brazil.
A total of 209 Vibrio were isolated. The most prevalent species was Vibrio parahaemolyticus (44.66%)
followed by Vibrio alginolyticus (19.62%) and Vibrio vulnificus (12.44%). All 209 Vibrio isolates tested
positive for the RNA polymerase alpha gene (rpoA). The tlh gene (thermolabile hemolysin), a genetic
marker for V. parahaemolyticus, and vvhA (cytolysin hemolysin) of V. vulnificus were detected in 85 and
26 isolates, respectively. The MALDI-TOF MS proteomic technique was used to confirm the
identification of the 41 V. alginolyticus isolates. Our most important finding was the detection of the tdh
virulence gene in 68.20% (58/85) of V. parahaemolyticus environmental strains. Besides the circulation
of the virulence gene, the spread of antimicrobial resistance was evaluated and 91.3% (191/209) of the
isolates showed resistance to ampicillin, 23.9% (50/209) to ciprofloxacin, 18.6% (39/209) to
nitrofurantoin, 5.7% (12/209) to tetracycline, 4.3% (9/209) to pefloxacin and 3.3% (7/209) to
chloramphenicol. These findings indicate that environmental isolates can act as reservoirs of virulence
and antibiotic resistance genes.