Acoustic communication in the form of courtship and mating songs are often involved in reproductive isolation between species of Diptera, such as Drosophila, mosquitoes and sand fies. The patterns of courtship songs in New World sand fy species evolve quickly under sexual selection; and therefore, represent an important trait that can be used as a marker to study the evolution of species complexes and may aid identifcation of sibling species with a complex. The ability to identify vector species within species complexes is of critical importance for efective and efcient vector control programs. Speciesspecifc song patterns seems to contribute to reproductive isolation in New World sand fy species, suggesting that auditory communication signals may be widespread among these important vectors of leishmaniasis. The main goal of the present study was to characterize the copulatory courtship song of Phlebotomus argentipes, an important vector of visceral leishmaniasis in the Old World. Ph. argentipes males produce acoustic signals during copulation and two types of songs were observed. The one we called primary song is a ‘pulse song’ with similar length and amplitude to the previously observed ‘P1’ pattern recorded in Brazilian populations of Lu. longipalpis s.l. The secondary song has ‘sine song’ characteristics and is quite diferent from any song produced by New World species. The discovery of this copulation courtship songs in Ph. argentipes supports the possibility that acoustic communication in sandfies might be more widespread than previously thought, including Old World species. Our results highlight the importance of further research on acoustic communication in the Ph. argentipes species complex and other Old World vectors of leishmaniasis.