Genomic Characterization of a Brazilian TT Virus Isolate Closely Related to SEN Virus-F

dc.creatorMendes, Leonardo Diniz
dc.creatorDevalle, Sylvie
dc.creatorNiel, Christian
dc.date2019-10-26T18:04:18Z
dc.date2019-10-26T18:04:18Z
dc.date2004
dc.date.accessioned2023-09-26T23:10:35Z
dc.date.available2023-09-26T23:10:35Z
dc.identifierMENDES, Leonardo Diniz; DEVALLE, Sylvie; NIEL, Christian. Genomic Characterization of a Brazilian TT Virus Isolate Closely Related to SEN Virus-F. Memórias do Insituto Oswaldo Cruz, Rio de Janeiro, v. 99. n. 3, p. 301-306, May 2004.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/36722
dc.identifier10.1590/S0074-02762004000300011
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8887682
dc.descriptionSEN virus (SENV) is a circular, single stranded DNA virus that has been first characterized in the serum of a human immunodeficiency virus type 1 (HIV-1)-infected patient. Eight genotypes of SENV (A-H) have been identified and further recognized as variants of TT virus (TTV) in the family Circoviridae. Here we describe the first genomic characterization of a SENV isolate (5-A) from South America. Using ‘universal’ primers, able to amplify most, if not all, TTV/SENV genotypes, a segment of > 3 kb was amplified by polymerase chain reaction from the serum of an HIV-1 infected patient. The amplicon was cloned and a 3087-nucleotide sequence was determined, that showed a high (85%) homology with the sequence of the Italian isolate SENV-F. Proteins encoded by open reading frames (ORFs) 1 to 4 consisted of 758, 129, 276, and 267 amino acids, respectively. By phylogenetic analysis, isolate 5-A was classified into TTV genotype 19 (phylogenetic group 3), together with SENV-F and TTV isolate SAa-38.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectHepatite
dc.subjectVírus da imunodeficiência humana tipo 1
dc.subjectSequenciamento de nucleotídeos
dc.subjectTorque teno virus
dc.subjectHepatitis
dc.subjectHuman immunodeficiency virus type 1
dc.subjectNucleotide sequencing
dc.subjectOpen reading frames 1-4
dc.subjectSEN virus-F
dc.subjectTT virus
dc.titleGenomic Characterization of a Brazilian TT Virus Isolate Closely Related to SEN Virus-F
dc.typeArticle


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