Article
Applicability of oral fluid samples for tracking hepatitis B virus mutations, genotyping, and phylogenetic analysis
Registro en:
PROTILHO, Moyra Machado et al. Applicability of oral fluid samples for tracking hepatitis B virus mutations, genotyping, and phylogenetic analysis. Archives of Virology, v. 166, p. 2435-2442, p. 1-8, 2021.
0304-8608
10.1007/s00705-021-05122-x
1452-8798
Autor
Portilho, Moyra Machado
Bezerra, Cristianne Sousa
Mendonça, Ana Carolina da Fonseca
Marques, Vanessa Alves
Nabuco, Leticia Cancella
Nogueira, Cristiane Alves Villela
Ivantes, Cláudia Alexandra Pontes
Lewis‑Ximenez, Lia Laura
Lago, Barbara Vieira do
Villar, Livia Melo
Resumen
aim of this study was to evaluate the usefulness of oral fluid samples to determine HBV genotype distribution, S/polymerase
mutations, and HBV subpopulation diversity among chronically HBV-infected individuals. Serum and oral fluid samples were
obtained from 18 individuals for PCR and nucleotide sequencing of the HBV surface antigen gene. Biochemical analysis of
liver enzymes (ALT, AST, GGT) and HBV, HCV, and HIV serological tests were also performed. All serum samples were
HBsAg (+), anti-HBc (+), and anti-HBs (−); 55.6% were HBeAg (+)/anti-HBe (−), and 11.1% were anti-HIV (+). The mean
HBV DNA viral load was 6.1 ± 2.3 log IU/mL. The HBV genotype distribution was as follows: A, 72.2%; D, 11.1%; E, 5.6%;
F, 11.1%. A concordance of 100% in genotype classification and 99.8% in sequence similarity between paired oral fluid and
serum samples was observed. HBsAg mutations were detected in all samples, but no resistance mutations were found in the
polymerase gene. This study demonstrates that oral fluid samples can be used reliably for tracking HBV mutations, genotyping,
and phylogenetic analysis. This could be important for molecular epidemiology studies with hard-to-reach populations. 2023