Article
Gut Microbiome Profiles and Associated Metabolic Pathways in HIV-Infected Treatment-Naïve Patients
Registro en:
NASCIMENTO, Wellinton M. do et al. Gut Microbiome Profiles and Associated Metabolic Pathways in HIV-Infected Treatment-Naïve Patients. Cells, v. 10, n. 385, 15p, 2021.
2073-4409
10.3390/cells10020385
Autor
Nascimento, Wellinton M. do
Machiavelli, Aline
Ferreira, Luiz G. E.
Silveira, Luisa Cruz
Azevedo, Suwellen S. D. de
Bello, Gonzalo
Smith, Daniel P.
Mezzari, Melissa P.
Petrosino, Joseph F.
Duarte, Rubens Tadeu Delgado
Zárate-Bladés, Carlos R.
Pinto, Aguinaldo R.
Resumen
The normal composition of the intestinal microbiota is a key factor for maintaining healthy
homeostasis, and accordingly, dysbiosis is well known to be present in HIV-1 patients. This article
investigates the gut microbiota profile of antiretroviral therapy-naive HIV-1 patients and healthy
donors living in Latin America in a cohort of 13 HIV positive patients (six elite controllers, EC, and
seven non-controllers, NC) and nine healthy donors (HD). Microbiota compositions in stool samples
were determined by sequencing the V3-V4 region of the bacterial 16S rRNA, and functional prediction
was inferred using PICRUSt. Several taxa were enriched in EC compared to NC or HD groups,
including Acidaminococcus, Clostridium methylpentosum, Barnesiella, Eubacterium coprostanoligenes, and
Lachnospiraceae UCG-004. In addition, our data indicate that the route of infection is an important
factor associated with changes in gut microbiome composition, and we extend these results by
identifying several metabolic pathways associated with each route of infection. Importantly, we
observed several bacterial taxa that might be associated with different viral subtypes, such as
Succinivibrio, which were more abundant in patients infected by HIV subtype B, and Streptococcus
enrichment in patients infected by subtype C. In conclusion, our data brings a significant contribution
to the understanding of dysbiosis-associated changes in HIV infection and describes, for the first
time, differences in microbiota composition according to HIV subtypes. These results warrant further
confirmation in a larger cohort of patients.