Defining the directionality and quality of influenza virus-specific CD8+ T cell cross-reactivity in individuals infected with hepatitis C virus

dc.creatorKasprowicz, Victoria
dc.creatorWard, Scott M.
dc.creatorTurner, Alison
dc.creatorGrammatikos, Alexandros
dc.creatorNolan, Brian E.
dc.creatorLewis-Ximenez, Lia
dc.creatorSharp, Charles
dc.creatorWoodruff, Jenny
dc.creatorFleming, Vicki M.
dc.creatorSims, Stuart
dc.creatorWalker, Bruce D.
dc.creatorSewell, Andrew K.
dc.creatorLauer, Georg M.
dc.creatorKlenerman, Paul
dc.date2019-02-26T14:51:06Z
dc.date2019-02-26T14:51:06Z
dc.date2008
dc.date.accessioned2023-09-26T23:02:48Z
dc.date.available2023-09-26T23:02:48Z
dc.identifierKASPROWICZ, Victoria; et al. Defining the directionality and quality of influenza virus–specific CD8+ T cell cross-reactivity in individuals infected with hepatitis C virus. Journal of Clinical Investigation, v.118, n.3, p.1143-1153, Mar. 2008.
dc.identifier0021-9738
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31880
dc.identifier10.1172/JCI33082
dc.identifier1558-8238
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8886217
dc.descriptionCross-reactivity of murine and recently human CD8(+) T cells between different viral peptides, i.e., heterologous immunity, has been well characterized. However, the directionality and quality of these cross-reactions is critical in determining their biological importance. Herein we analyzed the response of human CD8(+) T cells that recognize both a hepatitis C virus peptide (HCV-NS3) and a peptide derived from the influenza neuraminidase protein (Flu-NA). To detect the cross-reactive CD8(+) T cells, we used peptide-MHC class I complexes (pMHCs) containing a new mutant form of MHC class I able to bind CD8 more strongly than normal MHC class I complexes. T cell responses against HCV-NS3 and Flu-NA peptide were undetectable in normal donors. In contrast, some responses against the Flu-NA peptide were identified in HCV(+) donors who showed strong HCV-NS3-specific reactivity. The Flu-NA peptide was a weak agonist for CD8(+) T cells in HCV(+) individuals on the basis of novel pMHCs and functional assays. These data support the idea of cross-reactivity between the 2 peptides, but indicate that reactivity toward the Flu-NA peptide is highly CD8-dependent and occurs predominantly after priming during HCV infection. Our findings indicate the utility of the novel pMHCs in dissecting cross-reactivity and suggest that cross-reactivity between HCV and influenza is relatively weak. Further studies are needed to relate affinity and functionality of cross-reactive T cells.
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society for Clinical Investigation
dc.rightsopen access
dc.subjectVírus Influenza
dc.subjectCD+8
dc.subjectCélulas T
dc.subjectIndivíduos infectados
dc.subjectVírus da hepatite C
dc.subjectInfluenza virus
dc.subjectCD+8 specific
dc.subjectT cells
dc.subjectIndividuals infected
dc.subjectHepatitis C virus
dc.titleDefining the directionality and quality of influenza virus-specific CD8+ T cell cross-reactivity in individuals infected with hepatitis C virus
dc.typeArticle


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