dc.creator | Kasprowicz, Victoria | |
dc.creator | Ward, Scott M. | |
dc.creator | Turner, Alison | |
dc.creator | Grammatikos, Alexandros | |
dc.creator | Nolan, Brian E. | |
dc.creator | Lewis-Ximenez, Lia | |
dc.creator | Sharp, Charles | |
dc.creator | Woodruff, Jenny | |
dc.creator | Fleming, Vicki M. | |
dc.creator | Sims, Stuart | |
dc.creator | Walker, Bruce D. | |
dc.creator | Sewell, Andrew K. | |
dc.creator | Lauer, Georg M. | |
dc.creator | Klenerman, Paul | |
dc.date | 2019-02-26T14:51:06Z | |
dc.date | 2019-02-26T14:51:06Z | |
dc.date | 2008 | |
dc.date.accessioned | 2023-09-26T23:02:48Z | |
dc.date.available | 2023-09-26T23:02:48Z | |
dc.identifier | KASPROWICZ, Victoria; et al. Defining the directionality and quality of influenza virus–specific CD8+ T cell cross-reactivity in individuals infected with hepatitis C virus. Journal of Clinical Investigation, v.118, n.3, p.1143-1153, Mar. 2008. | |
dc.identifier | 0021-9738 | |
dc.identifier | https://www.arca.fiocruz.br/handle/icict/31880 | |
dc.identifier | 10.1172/JCI33082 | |
dc.identifier | 1558-8238 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8886217 | |
dc.description | Cross-reactivity of murine and recently human CD8(+) T cells between different viral peptides, i.e., heterologous immunity, has been well characterized. However, the directionality and quality of these cross-reactions is critical in determining their biological importance. Herein we analyzed the response of human CD8(+) T cells that recognize both a hepatitis C virus peptide (HCV-NS3) and a peptide derived from the influenza neuraminidase protein (Flu-NA). To detect the cross-reactive CD8(+) T cells, we used peptide-MHC class I complexes (pMHCs) containing a new mutant form of MHC class I able to bind CD8 more strongly than normal MHC class I complexes. T cell responses against HCV-NS3 and Flu-NA peptide were undetectable in normal donors. In contrast, some responses against the Flu-NA peptide were identified in HCV(+) donors who showed strong HCV-NS3-specific reactivity. The Flu-NA peptide was a weak agonist for CD8(+) T cells in HCV(+) individuals on the basis of novel pMHCs and functional assays. These data support the idea of cross-reactivity between the 2 peptides, but indicate that reactivity toward the Flu-NA peptide is highly CD8-dependent and occurs predominantly after priming during HCV infection. Our findings indicate the utility of the novel pMHCs in dissecting cross-reactivity and suggest that cross-reactivity between HCV and influenza is relatively weak. Further studies are needed to relate affinity and functionality of cross-reactive T cells. | |
dc.format | application/pdf | |
dc.language | eng | |
dc.publisher | American Society for Clinical Investigation | |
dc.rights | open access | |
dc.subject | Vírus Influenza | |
dc.subject | CD+8 | |
dc.subject | Células T | |
dc.subject | Indivíduos infectados | |
dc.subject | Vírus da hepatite C | |
dc.subject | Influenza virus | |
dc.subject | CD+8 specific | |
dc.subject | T cells | |
dc.subject | Individuals infected | |
dc.subject | Hepatitis C virus | |
dc.title | Defining the directionality and quality of influenza virus-specific CD8+ T cell cross-reactivity in individuals infected with hepatitis C virus | |
dc.type | Article | |